지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제8호
- 발행연도
- 2015.1
- 수록면
- 1,281 - 1,290 (10page)
이용수
초록· 키워드
오류제보하기
Thermolysin and its homologs are a group of metalloproteases that have been widely used in both therapeutic and biotechnological applications. We here report the identification and characterization of a novel thermolysin-like protease, BtsTLP1, from insect pathogen Bacillus thuringiensis serovar Sichuansis strain MC28. BtsTLP1 is extracellularly produced in Bacillus subtilis, and the active protein was purified via successive chromatographic steps. The mature form of BtsTLP1 has a molecule mass of 35.6 kDa as determined by mass spectrometry analyses. The biochemical characterization indicates that BtsTLP1 has an apparent Km value of 1.57 mg/ml for azocasein and is active between 20oC and 80oC. Unlike other reported neutral gram-positive thermolysin homologs with optimal pH around 7, BtsTLP1 exhibits an alkaline pH optimum around 10. The activity of BtsTLP1 is strongly inhibited by EDTA and a group of specific divalent ions, with Zn2+ and Cu2+ showing particular effects in promoting the enzyme autolysis. Furthermore, our data also indicate that BtsTLP1 has potential in cleaning applications.
목차
등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
Adekoya OA / 2009 / The thermolysin family (M4) of enzymes: therapeutic and biotechnological potential / Chem. Biol. Drug Des 73 : 7 ~ 16
Beaufort N / 2011 / The thermolysin-like metalloproteinase and virulence factor LasB from pathogenic Pseudomonas aeruginosa induces anoikis of human vascular cells / Cell Mic
Chung MC / 2006 / Secreted neutral metalloproteases of Bacillus anthracis as candidate pathogenic factors / J. Biol. Chem 281 : 31408 ~ 31418
Colman PM / 1972 / The structure of thermolysin: an electron density map at 2-3 A resolution / J. Mol. Biol 70 : 701 ~ 724
Fassina G / 1986 / Autolysis of thermolysin. Isolation and characterization of a folded three-fragment complex / Eur. J. Biochem 156 : 221 ~ 228
Beaufort N / 2011 / The thermolysin-like metalloproteinase and virulence factor LasB from pathogenic Pseudomonas aeruginosa induces anoikis of human vascular cells / Cell Mic
Chung MC / 2006 / Secreted neutral metalloproteases of Bacillus anthracis as candidate pathogenic factors / J. Biol. Chem 281 : 31408 ~ 31418
Colman PM / 1972 / The structure of thermolysin: an electron density map at 2-3 A resolution / J. Mol. Biol 70 : 701 ~ 724
Fassina G / 1986 / Autolysis of thermolysin. Isolation and characterization of a folded three-fragment complex / Eur. J. Biochem 156 : 221 ~ 228
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
호알칼리성 Bacillus sp. DK1122 균주가 생산하는 알칼리성 단백질 분해효소의 정제 및 특성
한국미생물·생명공학회지
2016 .01
-
Complete genome sequence of Bacillus thuringiensis C25, a potential biocontrol agent for sclerotia-forming fungal phytopathogens
미생물학회지
2017 .09
-
Distribution of Toxin Genes and Enterotoxins in Bacillus thuringiensis Isolated from Microbial Insecticide Products
Journal of Microbiology and Biotechnology
2015 .01
-
김치로부터 단백질 분해 효소활성이 우수한 Bacillus subtilis 균주의 분리
한국미생물·생명공학회지
2017 .01
-
Identification of a Newly Isolated Protease-producing Bacterium, Bacillus subtilis FBL-1, from Soil
한국미생물·생명공학회지
2016 .01
-
Alkaline Protease Production from Bacillus gibsonii 6BS15-4 Using Dairy Effluent and Its Characterization as a Laundry Detergent Additive
Journal of Microbiology and Biotechnology
2023 .02
-
Reliable Identification of Bacillus cereus Group Species Using Low Mass Biomarkers by MALDI-TOF MS
Journal of Microbiology and Biotechnology
2019 .01
-
Various Enterotoxin and Other Virulence Factor Genes Widespread Among Bacillus cereus and Bacillus thuringiensis Strains
Journal of Microbiology and Biotechnology
2015 .01
-
반응표면분석법을 이용한 Bacillus amyloliquefaciens SRCM115785의 protease 활성증가를 위한 배지 최적화
생명과학회지
2021 .08
-
Prevalence and Toxin Characteristics of Bacillus thuringiensis Isolated from Organic Vegetables
Journal of Microbiology and Biotechnology
2017 .01
-
Biochemical Characterization of a Novel Alkaline and Detergent Stable Protease from Aeromonas veronii OB3
한국미생물·생명공학회지
2020 .01
-
Purification and Characterization of a Novel Extracellular Thermostable Alkaline Protease from Streptomyces sp. M30
Journal of Microbiology and Biotechnology
2015 .01
-
Isolation of a Lipolytic and Proteolytic Bacillus licheniformis from Refinery Oily Sludge and Optimization of Culture Conditions for Production of the Enzymes
한국미생물·생명공학회지
2020 .01
-
Characterization of a Thermostable Alkaline Mannanase Purified from Bacillus subtilis subsp. inaquosorum CSB31
한국미생물학회 학술대회논문집
2019 .04
-
Screening and Fermentation Characteristics of Bacillus sp. with High Amylase and Protease Activity Isolated from Traditional Nuruks
한국미생물학회 학술대회논문집
2016 .04
-
Enzymatic Characterization of γ-Glutamyl Hydrolase from Bacillus licheniformis DSM 13 Heterologously Expressed in Bacillus subtilis
한국미생물학회 학술대회논문집
2020 .10
-
Identification and Characterization of a Bacteriocin from the Newly Isolated Bacillus subtilis HD15 with Inhibitory Effects against Bacillus cereus
Journal of Microbiology and Biotechnology
2022 .11
-
Complete genome sequence of Bacillus subtilis BS16045 isolated from Gochujang
미생물학회지
2017 .03
-
Identification of a Bacillus thuringiensis Surface Layer Protein with Cytotoxic Activity against MDA-MB-231 Breast Cancer Cells
Journal of Microbiology and Biotechnology
2017 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0