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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제10호
- 발행연도
- 2008.1
- 수록면
- 1,695 - 1,700 (6page)
이용수
초록· 키워드
오류제보하기
An immunosensor based on surface plasmon resonance (SPR) with enhanced performance was developed through a mixed self-assembled monolayer. A mixture of 16- mercaptohexadecanic acid (16-MHA) and 1-undecanethiol with various molar ratios was self-assembled on gold (Au) surface and the carboxylic acid groups of 16-MHA were then coordinated to Zn ions by exposing the substrate to an ethanolic solution of Zn(NO3)2·6H2O. The antibody was immobilized on the SPR surface by exposing the functionalized substrate to the desired solution of antibody in phosphatebuffered saline (PBS) molecules. The film formation in series was confirmed by SPR and atomic force microscopy (AFM). The functionalized surface was applied to develop an SPR immunosensor for detecting human serum albumin (HSA) and the estimated detection limit (DL) was 4.27 nM.
The limit value concentration can be well measured between ill and healthy conditions.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Alfredo de la E.-M. / 2006 / Determination of human serum albumin using aurothiomalate as electroactive label. Anal. Bioanal / Chem 384 : 742 ~ 750
Baek, S. H. / 2004 / Surface plasmon resonance imaging analysis of hexahistidine-tagged protein on the gold thin film coated with a calix crown derivative. Biotechnol / Biopr
Briand, E. / 2006 / Immobilization of protein A on SAMs for the elaboration of immunosensors. Colloids Surf / Biointerfaces 53 : 215 ~ 224
Darren, M. D. / 1998 / Covalent coupling of immunoglobulin G to self-assembled monolayers as a method for immobilizing the interfacial recognition layer of a surface plasmon
Dill, K. / 1997 / Rapid, sensitive and specific detection of whole cells and spores using the light-addressable potentiometric sensor. J. Biochem. Biophys / Methods 34 : 161
Baek, S. H. / 2004 / Surface plasmon resonance imaging analysis of hexahistidine-tagged protein on the gold thin film coated with a calix crown derivative. Biotechnol / Biopr
Briand, E. / 2006 / Immobilization of protein A on SAMs for the elaboration of immunosensors. Colloids Surf / Biointerfaces 53 : 215 ~ 224
Darren, M. D. / 1998 / Covalent coupling of immunoglobulin G to self-assembled monolayers as a method for immobilizing the interfacial recognition layer of a surface plasmon
Dill, K. / 1997 / Rapid, sensitive and specific detection of whole cells and spores using the light-addressable potentiometric sensor. J. Biochem. Biophys / Methods 34 : 161
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