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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제16권 제8호
- 발행연도
- 2006.1
- 수록면
- 1,169 - 1,173 (5page)
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초록· 키워드
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Imaging ellipsometry (IE) for detection of bindingof Escherichia coli O157:H7 (E. coli O 1 5 7 : H 7 ) t o a nimmunosensor is reported. A protein G layer, chemicalybound to a self-assembled layer of 11-mercaptoundecanoic acid(11-MUA), was adopted for immobilization of monoclonalantibody against E. coli O157:H7 (Mab). The immobilizationof antibody was investigated using surface plasmon resonance.To fabricate antibody spots on a gold surface, protein Gsolution was spoted onto the gold surface modified with an1-MUA layer, followed by immobilizing Mab on the proteinG spot. Elipsometric images of the protein G spot, the Mabspot, and Mab spots with binding of E. coli O157:H7 invarious concentrations were acquired using the IE system.ellipsometric images indicated that the lowest detection limitwas 103 CFU/ml for E. coli O157:H7. Thus, IE can be appliedto an immunosensor for detection of E. coli O157:H7 as adetection method with the advantages of alowing label-freedetection, high sensitivity, and operational simplicity.
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