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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국진공학회(ASCT) Applied Science and Convergence Technology Applied Science and Convergence Technology Vol.25 No.3
- 발행연도
- 2016.5
- 수록면
- 61 - 65 (5page)
이용수
초록· 키워드
오류제보하기
Here, the rapid detection of Salmonella typhimurium by a portable surface plasmon resonance (SPR) biosensor in which the beam from a diode laser is modulated by a rotating mirror is reported. Using this system, immunoassay based on lipopolysaccharides (LPS)-specific monoclonal anti-Salmonella antibody was performed. For the purpose of orientation-controlled immobilization of antibodies on the SPR chip surface, the cysteine-mediated immobilization method, which is based on interaction between a gold surface and a thiol group (-SH) of cysteine, was adopted. As a result, using the portable SPR-based immunoassay, we detected S. typhimurium in the range from 10^7 CFU/mL to 10^9 CFU/mL within 1 hour. The results indicate that the portable SPR system could be potentially applied for general laboratory detection as well as on-site monitoring of foodborne, clinical, and environmental agents of interest.
목차
Abstract
Ⅰ. Introduction
Ⅱ. Materials and Methods
Ⅲ. Results and Discussion
Ⅳ. Conclusions
References
참고문헌 (16)
참고문헌 신청
M. M. Arshad, M. J. Wilkins, F. P. Downes, M. H. Rahbar, R. J. Erskine, M. L. Boulton, and A. M. Saeed, Foodborne Pathog. Dis. 4, 16 (2007).
H. L. Alakomi, and M. Saarela, Qual. Assur. Saf. Crop. 1, 142e152 (2009).
K. G. Maciorowski, P. Herrera, F. T. Jones, S. D. Pillai, and S. C. Ricke, Vet. Res. Commun. 30, 127e137 (2006).
J. M. Eijkelkamp, H. J. M. Aarts, and H. J. van der Fels-Klerx, Food Anal. Method. 2, 1 (2009).
B. Malorny, J. Hoorfar, C. Bunge, and R. Helmuth, Appl. Environ. Microbiol. 69, 290 (2003).
H. L. Alakomi, and M. Saarela, Qual. Assur. Saf. Crop. 1, 142e152 (2009).
K. G. Maciorowski, P. Herrera, F. T. Jones, S. D. Pillai, and S. C. Ricke, Vet. Res. Commun. 30, 127e137 (2006).
J. M. Eijkelkamp, H. J. M. Aarts, and H. J. van der Fels-Klerx, Food Anal. Method. 2, 1 (2009).
B. Malorny, J. Hoorfar, C. Bunge, and R. Helmuth, Appl. Environ. Microbiol. 69, 290 (2003).
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UCI(KEPA) : I410-ECN-0101-2019-420-001276089