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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제2호
- 발행연도
- 2017.1
- 수록면
- 251 - 261 (11page)
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초록· 키워드
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Initially, we screened 18 Aspergillus sojae-like strains from Aspergillus spp. isolated from meju (Korean traditional fermented soybean brick) according to their morphological characteristics. Because members of Aspergillus section Flavi are often incorrectly identified because of their phylogenetic similarity, we re-identified these strains at the morphological and molecular genetic levels. Fourteen strains were finally identified as A. sojae. The isolates produced protease and α-amylase with ranges of 2.66-10.64 and 21.53-106.73 unit/g-initial dry substrate (U/g-IDS), respectively, which were equivalent to those of the koji (starter mold) strains employed to produce Japanese soy sauce. Among the isolates and Japanese koji strains, strains SMF 127 and SMF 131 had the highest leucine aminopeptidase (LAP) activities at 6.00 and 6.06 U/g-IDS, respectively. LAP plays an important role in flavor development because of the production of low-molecular-weight peptides that affect the taste and decrease bitterness. SMF 127 and SMF 131 appeared to be non-aflatoxigenic because of a termination point mutation in aflR and the lack of the polyketide synthase gene found in other A. sojae strains. In addition, SMF 127 and SMF 131 were not cyclopiazonic acid (CPA) producers because of the deletion of maoA, dmaT, and pks/nrps, which are involved in CPA biosynthesis. Therefore, A. sojae strains such as SMF 127 and SMF 131, which have high protease and LAP activities and are free of safety issues, can be considered good starters for soybean fermentations, such as in the production of the Korean fermented soybean products meju, doenjang, and ganjang.
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참고문헌 (24)
참고문헌 신청
Buyukkileci AO / 2011 / Enhanced production of exo-polygalacturonase from agrobased products by Aspergillus sojae / Bioresources 6 : 3452 ~ 3468
Chang H-Y / 2011 / Purification and characterisation of Aspergillus sojae naringinase: the production of prunin exhibiting markedly enhanced solubility with in vitro inhibit
Chang P-K / 2007 / Understanding nonaflatoxigenicity of Aspergillus sojae: a windfall of aflatoxin biosynthesis research / Appl. Microbiol. Biotechnol 76 : 977 ~ 984
Chang P-K. / 2004 / Lack of interaction between AFLR and AFLJ contributes to nonaflatoxigenicity of Aspergillus sojae / J. Biotechnol 107 : 245 ~ 253
Glass NL / 1995 / Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes / Appl. Environ. Microbiol 61 : 1323 ~ 133
Chang H-Y / 2011 / Purification and characterisation of Aspergillus sojae naringinase: the production of prunin exhibiting markedly enhanced solubility with in vitro inhibit
Chang P-K / 2007 / Understanding nonaflatoxigenicity of Aspergillus sojae: a windfall of aflatoxin biosynthesis research / Appl. Microbiol. Biotechnol 76 : 977 ~ 984
Chang P-K. / 2004 / Lack of interaction between AFLR and AFLJ contributes to nonaflatoxigenicity of Aspergillus sojae / J. Biotechnol 107 : 245 ~ 253
Glass NL / 1995 / Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes / Appl. Environ. Microbiol 61 : 1323 ~ 133
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