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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제26권 제11호
- 발행연도
- 2016.1
- 수록면
- 1,993 - 2,005 (13page)
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Bacillus pumilus is one of the most characterized microorganisms that are used for high-level production of select industrial enzymes. A novel B. pumilus SCU11 strain possessing high alkaline protease activity was obtained in our previous work. The culture supernatant of this strain showed efficient dehairing capability with minimal collagen damage, indicating promising potential applications in the leather industry. In this study, the strain’s extracellular proteome was identified by LC-MS/MS-based shotgun proteomic analysis, and their related secretory pathways were characterized by BLAST searches. A total of 513 proteins, including 100 actual secreted and 413 intracellular proteins, were detected in the extracellular proteome.
The functions of these secreted proteins were elucidated and four complete secretory systems (Sec, Tat, Com, and ABC transporter) were proposed for B. pumilus. These data provide B. pumilus a comprehensive extracellular proteome profile, which is a valuable theoretical and applicative basis for future genetic modifications and development of industrial enzymes.
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참고문헌 (56)
참고문헌 신청
Antelmann H / 2006 / Proteomic survey through secretome of Bacillus subtilis / Methods Biochem. Anal 49:179
Bottone EJ / 2003 / Production by Bacillus pumilus (MSH) of an antifungal compound that is active against Mucoraceae and Aspergillus species: preliminary report / J. Med. Microbiol 52:69~74
Britton RA / 2002 / Genome-wide analysis of the stationary-phase sigma factor (sigma-H)regulon of Bacillus subtilis / J. Bacteriol 184:4881~4890
Carroll RK / 2014 / The lone S41 family C-terminal p rocessing protease in Staphylococcus aureus is localized to the cell wall and contributes to virulence / Microbiology 160:1737~1748
Nack-Shick Choi / 2010 / Expression and Identification of a Minor Extracellular Fibrinolytic Enzyme (Vpr) from Bacillus subtilis KCTC 3014 / Biotechnology and Bioprocess Engineering 15(3):446~452
Bottone EJ / 2003 / Production by Bacillus pumilus (MSH) of an antifungal compound that is active against Mucoraceae and Aspergillus species: preliminary report / J. Med. Microbiol 52:69~74
Britton RA / 2002 / Genome-wide analysis of the stationary-phase sigma factor (sigma-H)regulon of Bacillus subtilis / J. Bacteriol 184:4881~4890
Carroll RK / 2014 / The lone S41 family C-terminal p rocessing protease in Staphylococcus aureus is localized to the cell wall and contributes to virulence / Microbiology 160:1737~1748
Nack-Shick Choi / 2010 / Expression and Identification of a Minor Extracellular Fibrinolytic Enzyme (Vpr) from Bacillus subtilis KCTC 3014 / Biotechnology and Bioprocess Engineering 15(3):446~452
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