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논문 기본 정보
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- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제19권 제1호
- 발행연도
- 2009.1
- 수록면
- 83 - 92 (10page)
이용수
초록· 키워드
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Individual immunochromatographic assays (ICG) for
ochratoxin A (OTA) and zearalenone (ZEA) were optimized
and used in the development of a one-step simultaneous
immunochromatographic assay (OS-ICG) for the rapid
multianalysis of two mycotoxins in corn samples. The
nitrocellulose membrane of the OS-ICG was treated with
OTA-bovine serum albumin (BSA), ZEA-ovalbumin (OVA),
and anti-mouse IgG in the OTA test, ZEA test, and control
zones, respectively. Monoclonal antibody-gold conjugates
(OTA3 MAb-gold and ZEA2C5 MAb-gold) were sprayed
onto the conjugate pad. The visual detection limits were
2.5 and 5 ng/ml for OTA and ZEA, resepectively, and the
results were obtained within 15 min after starting the
analysis. An efficient, simple, and rapid extraction method
using 30% MeOH/PBS was established and validated by
analyzing the corn samples spiked with OTA/ZEA mixtures
(0/0, 5/10, 10/20, and 20/30 µg/kg). The cut-off values of
the OS-ICG for the spiked corn were 5 and 10 µg/kg for
OTA and ZEA, respectively. Natural corn samples were
analyzed by OS-ICG, direct competitive enzyme-linked
immunosorbent assay (DC-ELISA), and HPLC. Results of
the OS-ICG were in good agreement with those obtained
by DC-ELISA and HPLC. The developed OS-ICG offers
a rapid, easy-to-use, and portable analytical system and
can be used as a convenient qualitative tool for the on-site
simultaneous determination of OTA and ZEA in cereals,
food, and agricultural products in one analytical cycle.
목차
등록된 정보가 없습니다.
참고문헌 (20)
참고문헌 신청
Andrea, D. / 1999 / Stabilization of enzyme immunoassays for atrazine / Anal. Chim. Acta 362 : 35 ~ 45
Cho, Y. J. / 2005 / Production of a monoclonal antibody against ochratoxin A and its application to immunochromatographic assay / J. Agric. Food Chem 53 : 8447 ~ 8451
Domijan, A. M. / 2005 / Fumonisin B1, fumonisin B2, zearalenone and ochratoxin A contamination of maize in Croatia / Food Addit. Contam 22 : 677 ~ 680
European Commission / 2002 / Commission Directive 2002/26/ EC of 13 March 2002 laying down the sampling methods and the methods of analysis for the official control of the le
European Commission / 2006 / Commission Regulation 1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs / Off. J. Eur. Union L 364 : 5
Cho, Y. J. / 2005 / Production of a monoclonal antibody against ochratoxin A and its application to immunochromatographic assay / J. Agric. Food Chem 53 : 8447 ~ 8451
Domijan, A. M. / 2005 / Fumonisin B1, fumonisin B2, zearalenone and ochratoxin A contamination of maize in Croatia / Food Addit. Contam 22 : 677 ~ 680
European Commission / 2002 / Commission Directive 2002/26/ EC of 13 March 2002 laying down the sampling methods and the methods of analysis for the official control of the le
European Commission / 2006 / Commission Regulation 1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs / Off. J. Eur. Union L 364 : 5
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