지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제15권 제4호
- 발행연도
- 2005.1
- 수록면
- 722 - 727 (6page)
이용수
초록· 키워드
오류제보하기
Cytosolic sialidase (Neu2), a member of thesialidase family that is responsible for hydrolysis of sialic acidfrom the terminal position of sialoglycoconjugates, is poorlyexpressed in skeletal muscle and not detected in any otheradult tissues. Thus, we isolated Neu2 cDNA using splicing byoverlap extension (SOEing). In order to further characterize thisenzyme, a His-tagged derivative was expressed in the bacterialexpression system and purified by Ni2+-affinity chromatography.A recombinant product of approximately 42 kDa had sialidaseactivity toward 4-methyl-umbelliferyl-α-D-N-acetylneuraminicacid (4MU-NeuAc). The optimal pH and temperature of therecombinant Neu2 for 4MU-NeuAc was 6.0 and 37.5oC,respectively. The metal ions, such as Cu2+ and Cd2+, showedstrong inhibitory effect on the activity of the enzyme. Theenzyme efficiently hydrolyzed the gangliosides GM3 andGD3 and had relatively low activities on ganglioside GD1aand GD1b, α2-3 sialyllactose, and sialylated glycoproteinssuch as fetuin, transferrin, and orsomucoid, but had hardlyany activities on α2-6 sialyllactose and ganglioside GM1 andGM2. We concluded that the recombinant Neu2 has a sialidaseactivity toward glycoproteins as well as gangliosides.
목차
등록된 정보가 없습니다.
참고문헌 (28)
참고문헌 신청
Chung / 2003 / Salmonellatyphimurium LPS confers its resistance to antibacterial agentsof baicalin of Scutellaria baicalensis George and Novomycin Complementation of the rfaE
Chung / 2005 / Molecular mechanism for transcriptional activation ofganglioside GM3 synthase and its function in differentiationof HL-60 cells 15 : 233 ~ 15 244
Corfield,T / 1992 / Bacterial sialidases:Roles in pathogenicityand nutrition
Ferrari / 1994 / Cloning andexpression of a soluble sialidase from Chinese hamster ovarycells Sequence alignment similarities to bacterial sialidases : 367 ~ 4 373
Garman / 1993 / Crystal structure of a bacterial sialidase : 9852 ~ 90 9856
Chung / 2005 / Molecular mechanism for transcriptional activation ofganglioside GM3 synthase and its function in differentiationof HL-60 cells 15 : 233 ~ 15 244
Corfield,T / 1992 / Bacterial sialidases:Roles in pathogenicityand nutrition
Ferrari / 1994 / Cloning andexpression of a soluble sialidase from Chinese hamster ovarycells Sequence alignment similarities to bacterial sialidases : 367 ~ 4 373
Garman / 1993 / Crystal structure of a bacterial sialidase : 9852 ~ 90 9856
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Phosphatidylinositol 4-phosphate 5-kinase α is induced in ganglioside-stimulated brain astrocytes and contributes to inflammatory responses
Experimental and Molecular Medicine
2010 .01
-
Differential Distribution of Gangliosides in Adult Rat Oviduct.
한국동물학회 학술대회
1998 .01
-
Ganglioside GM3 as a modulator of cancer cell proliferation mediates enhanced expression of tumor suppressor PTEN
한국당과학회 학술대회
2006 .01
-
Differential expression patterns of gangliosides in the tissues and cells of NIH-mini pig kidneys
Animal cells and systems
2010 .01
-
Differential Expression Patterns of Gangliosides in the Liver and Heart of NIH-miniature Pigs
생명과학회지
2010 .04
-
Immunochemical Studies of Starfish Gangliosides : Production of Monoclonal Antibody against AG-2, the Major Ganglioside of Starfish Acanthaster planci, and Detecting Its Distribution in Tissues by TLC Immunostaining
한국해양바이오학회지
2006 .12
-
Roles of gangliosides in mouse embryogenesis and embryonic stem cell differentiation
Experimental and Molecular Medicine
2011 .01
-
Dynamic changes of gangliosides expression during thedifferentiation of embryonic and mesenchymal stem cellsinto neural cells
Experimental and Molecular Medicine
2006 .01
-
Ganglioside GM3 is involved in neuronal cell death
한국당과학회 학술대회
2006 .01
-
Genetic Structure in the Region Near the Sialidase Gene in Bacteroides fragilis
The Journal of the Korean Society for Microbiology
2000 .10
-
Protein kinase A mediates microglial activation induced by plasminogen and gangliosides
Experimental and Molecular Medicine
2004 .01
-
Sialic Acid 함량 증가 배양기술에 의한 재조합 인간 다당쇄 에리스로포이에틴의 생산
한국미생물·생명공학회지
2004 .01
-
Genetic Variation and Hemoglobin and Ganglioside Monooxygenase in Korean Sapsarees ( Canis familiaris )
Journal of Life Science
2000 .10
-
Cloning and DNA Sequencing of Cytosolic Cu/Zn Superoxide Dismutase Gene from Chinese Cabbage
식물학회지
1998 .01
-
Conformational Study of Hydroxy Protons in $G_{A1}$ by NMR Spectroscopy
한국생물물리학회 학술발표회
1996 .01
-
Soluble Production of CMP-Neu5Ac Synthetase by Co-expression of Chaperone Proteins in Escherichia coli
한국미생물·생명공학회지
2014 .01
-
T cell-specific promoter activity of the human NeuAc α2,3Galβ1,3GalNAcα2,6-sialyltransferase
한국생명과학회 심포지움
2003 .05
-
HER-2/neu Protein Expression in Canine Mammary Adenocarcinoma
생명과학회지
2008 .01
-
Relationship between ganglioside expression and anti-cancer effects of the monoclonal antibody against epithelial cell adhesion molecule in colon cancer
Experimental and Molecular Medicine
2011 .01
-
A Recombinant Mouse GM-CSF Protein Expressed as an Inclusion Form Shows Colony Stimulating Activity
The Journal of Microbiology
2000 .06
이 논문의 저자 정보
최근 본 자료
댓글(0)
0