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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제14권 제2호
- 발행연도
- 2004.1
- 수록면
- 297 - 301 (5page)
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The dTDP-D-glucose 4,6-dehydratase fromSalmonella enterica was immobilized using covalent bindingto cyanogen bromide activated sepharose. The immobilizedenzyme was used to produce dTDP-4-keto-6-deoxy-D-glucose,a key sugar intermediate that can be used economically toproduce diverse classes of unusual sugars appended in variousantibiotics. The enzyme was immobilized on the sepharose afteractivation with cyanogen bromide. The maximum immobilization(80.03%) was achieved after 14 h of coupling. The covalentlyimmobilized enzyme was stable, and an average of 78.4%conversion was achieved until 120 h of immobilization whenit was repeatedly used. Similar conversion was noticed for thefirst batch using the enzyme entrapped-hydrogel but activitywas gradually decreased in the following batches. Theproduction of dTDP-4-keto-6-deoxy-D-glucose by using animmobilized enzyme has high potential for commercialapplication.
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참고문헌 (23)
참고문헌 신청
Allard / 2001 / The crystal structureof dTDP-D-glucose 4,6-dehydratase (RmlB) from Salmonellaenterica, the second enzyme in the dTDP-L-rhamnosepathway 307 : 283 ~ 295
Austin / 1993 / Purification of aPasturella haemolytica serotype 1-specific polysaccharideepitope by use of monoclonal antibody immunoaffinity : 695 ~ 700
Bickerstaff / 1997 / Immobilization of Enzymes andCells
Bradford / 1976 / A rapid and sensitive method for thequantitation of microgram quantities of protein utilizing theprinciple of protein-dye binding : 248 ~ 254
Chen / 2000 / Deoxy sugarsin glycopeptide antibiotics: Enzymatic synthesis of TDPL-epivancosamine in chloroeremomycin biosynthesis 97 : 11942 ~ 11947
Austin / 1993 / Purification of aPasturella haemolytica serotype 1-specific polysaccharideepitope by use of monoclonal antibody immunoaffinity : 695 ~ 700
Bickerstaff / 1997 / Immobilization of Enzymes andCells
Bradford / 1976 / A rapid and sensitive method for thequantitation of microgram quantities of protein utilizing theprinciple of protein-dye binding : 248 ~ 254
Chen / 2000 / Deoxy sugarsin glycopeptide antibiotics: Enzymatic synthesis of TDPL-epivancosamine in chloroeremomycin biosynthesis 97 : 11942 ~ 11947
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