지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제14권 제2호
- 발행연도
- 2004.1
- 수록면
- 417 - 421 (5page)
이용수
초록· 키워드
오류제보하기
A normal prion protein (PrPc) is converted to aprotease resistant isoform (PrPsc) by an apparent selfpropagatingactivity in bovine spongiform encephalopathies(BSE), which is a neurodegenerative disease. The cDNAencoding bovine PrP open reading frame (ORF) in Koreancattle was cloned by polymerase chain reaction (PCR). Thecloned cDNA had a length of 795 base pairs which coded fora protein of 264 amino acid residues with a calculatedmolecular mass of 28.6 kDa. Identities of 90, 90, 79 and 78%on nucleotide and 94, 94, 84, and 84% on amino acidsequence were shown to PrP genes from sheep, goat, human,and mouse, respectively. The cloned DNA was ligated intothe pQE30 expression vector and transformed into E. coliM15. The PrP was expressed by induction with isopropyl-b-D-thiogalactoside (IPTG) and purified on the Ni-NTA affinitycolumn. High specific activities of the recombinant PrP wereobserved in the fraction of pH 5.8 eluate and showed amolecular mass of ~29 kDa on SDS-PAGE and Western blotanalysis.
목차
등록된 정보가 없습니다.
참고문헌 (25)
참고문헌 신청
Anderson / 1996 / Transmission dynamic andepidemiology of BSE in British cattle 382 : 779 ~ 382 788
Basler / 1986 / Scrapie and cellular PrP isoforms areencoded by the same chromosomal gene : 417 ~ 428
Caughey / 1988 / Detectionof prion protein mRNA in normal and scrapie-infectedtissues and cell lines : 711 ~ 716
Chesebro / 1986 / Molecular cloning and complete sequence of prion proteincDNA from mouse brain infected with the scrapie agent : 6372 ~ 83 6376
Corsaro / 2002 / Expression in E coli and purification of recombinantfragments of wild type and mutant human prion protein 41 : 55 ~ 63
Basler / 1986 / Scrapie and cellular PrP isoforms areencoded by the same chromosomal gene : 417 ~ 428
Caughey / 1988 / Detectionof prion protein mRNA in normal and scrapie-infectedtissues and cell lines : 711 ~ 716
Chesebro / 1986 / Molecular cloning and complete sequence of prion proteincDNA from mouse brain infected with the scrapie agent : 6372 ~ 83 6376
Corsaro / 2002 / Expression in E coli and purification of recombinantfragments of wild type and mutant human prion protein 41 : 55 ~ 63
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Comparative bioinformatics analysis of prion proteins isolatedfrom reptile, rodent, ruminant, and human species
Experimental and Molecular Medicine
2007 .01
-
Mycosporine-like amino acids (MAAs) 처리에 따른 배양세포 내스크래피 프리온 단백질의 형성증가
한국미생물·생명공학회지
2015 .01
-
Biochemical Analysis of Interaction between Kringle Domains of Plasminogen and Prion Proteins with Q167R Mutation
Journal of Microbiology and Biotechnology
2017 .01
-
High-Level Production of High-Purity Human and Murine Recombinant Prion Proteins Functionally Compatible to In Vitro Seeding Assay
Journal of Microbiology and Biotechnology
2018 .01
-
Discriminant analysis of prion sequences for prediction of susceptibility
Experimental and Molecular Medicine
2013 .01
-
Prions and Prion Diseases: Fundamentals and Mechanistic Details
Journal of Microbiology and Biotechnology
2007 .01
-
Characterizing affinity epitopes between prion protein and b-amyloid using an epitope mapping immunoassay
Experimental and Molecular Medicine
2013 .01
-
Enhanced skin wound healing by a sustained release of growth factors contained in platelet-rich plasma
Experimental and Molecular Medicine
2011 .01
-
Prion Diseases: from Genetics to Pathogenic Mechanisms
한국미생물학회 학술대회논문집
2009 .05
-
새로운 벡터 pRP7을 이용한 난분해성 방향족 화합물 분해세균의 분자육종
미생물학회지
1994 .12
-
Decrease of Protease-Resistant PrPSc Level in ScN2a Cells by Polyornithine and Polyhistidine
Journal of Microbiology and Biotechnology
2018 .01
-
Gene Expressions of Cytokines and Increased NF-κ B Activity in the Brain of 87V Prion-Infected Mouse
한국생명과학회 학술발표회
1998 .01
-
GLOBAL CONVERGENCE OF A NEW SPECTRAL PRP CONJUGATE GRADIENT METHOD
Journal of applied mathematics & informatics
2011 .01
-
Platelet-rich plasma inhibits the apoptosis of highly adipogenic homogeneous preadipocytes in an in vitro culture system
Experimental and Molecular Medicine
2012 .01
-
Purification and Cloning of o Protein Secreted from Lactobacillus acidophilus
Korean journal of biological sciences
1998 .01
-
THE UNUSUAL INFECTIOUS PATHOGEN, PRION
한국미생물학회 학술대회논문집
1997 .04
-
Partial Sequence Analysis of Randomly Selected Watermelon [Citrullus lanantus (Thunb.) Mansf.] cDNA Clones
식물학회지
1997 .01
-
Production of Mannose-Binding Protein cDNA from Rat Liver and Recombinant Protein using a Bacterial Expression System
한국생명과학회 심포지움
2006 .09
-
Characterization and Functional Analysis of Antifungal Protein cDNA Clone from hot Pepper
한국동물학회 학술대회
1999 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0