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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제28권 제4호
- 발행연도
- 2018.1
- 수록면
- 571 - 578 (8page)
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Biofuel production using lignocellulosic biomass is gaining attention because it can be substituted for fossil fuels without competing with edible resources. However, because Saccharomyces cerevisiae does not have a D-xylose metabolic pathway, oxidoreductase or isomerase pathways must be introduced to utilize D-xylose from lignocellulosic biomass in S. cerevisiae. To elucidate the biochemical properties of xylose isomerase (XI) from Piromyces sp. E2 (PsXI), we determine its crystal structure in complex with substrate mimic glycerol. An amino- acid sequence comparison with other reported XIs and relative activity measurements using five kinds of divalent metal ions confirmed that PsXI belongs to class II XIs. Moreover kinetic analysis of PsXI was also performed using Mn2+, the preferred divalent metal ion for PsXI. In addition, the substrate-binding mode of PsXI could be predicted with the substrate mimic glycerol bound to the active site. These studies may provide structural information to enhance D-xylose utilization for biofuel production.
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참고문헌 (30)
참고문헌 신청
Sims RE / 2010 / An overview of second generation biofuel technologies / Bioresour. Technol. 101:1570~1580
Jonsson LJ / 2013 / Bioconversion of lignocellulose: inhibitors and detoxification / Biotechnol. Biofuels 6:16
Metzger JO / 2009 / Sustainable global energy supply based on lignocellulosic biomass from afforestation of degraded areas / Naturwissenschaften 96:279~288
Lee SM / 2014 / Systematic and evolutionary engineering of a xylose isomerase-based pathway in Saccharomyces cerevisiae for efficient conversion yields / Biotechnol. Biofuels 7:122
Van Vleet JH / 2009 / Yeast metabolic engineering for hemicellulosic ethanol production / Curr. Opin. Biotechnol. 20:300~306
Jonsson LJ / 2013 / Bioconversion of lignocellulose: inhibitors and detoxification / Biotechnol. Biofuels 6:16
Metzger JO / 2009 / Sustainable global energy supply based on lignocellulosic biomass from afforestation of degraded areas / Naturwissenschaften 96:279~288
Lee SM / 2014 / Systematic and evolutionary engineering of a xylose isomerase-based pathway in Saccharomyces cerevisiae for efficient conversion yields / Biotechnol. Biofuels 7:122
Van Vleet JH / 2009 / Yeast metabolic engineering for hemicellulosic ethanol production / Curr. Opin. Biotechnol. 20:300~306
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