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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제26권 제7호
- 발행연도
- 2016.1
- 수록면
- 1,252 - 1,258 (7page)
이용수
초록· 키워드
오류제보하기
1,3-Propanediol (1,3-PD) is a valuable platform compound. Many studies have shown that the supplement of NADH plays a key role in the bioproduction of 1,3-PD from Klebsiella pneumoniae. In this study, the xylA and xylB genes from Escherichia coli were overexpressed individually or simultaneously in K. pneumoniae to improve the production of 1,3-PD by cofermentation of glycerol and xylose. Compared with the parent strain, the xylose consumption was significantly increased by the introduction of these two genes. The 1,3-PD titers were raised from 17.9 g/l to 23.5, 23.9, and 24.4 g/l, respectively, by the overexpression of xylA and xylB as well as their coexpression. The glycerol conversion rate (mol/mol) was enhanced from 54.1% to 73.8%. The concentration of 2,3-butanediol was increased by 50% at the middle stage but drastically decreased after that. The NADH and NADH/NAD+ ratio were improved. This report suggests that overexpression of xylA or xylB is an effective strategy to improve the xylose assimilation rate to provide abundant reducing power for the biosynthesis of 1,3-PD in K. pneumoniae.
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참고문헌 (22)
참고문헌 신청
Abbad-Andaloussi S / 1995 / Isolation and characterization of Clostridium butyricum DSM 5431 mutants with increased resistance to 1,3-propanediol and altered production of acids / Appl. Environ. Microbiol 61:4413~4417
Ainala SK / 2013 / Glycerol assimilation and production of 1,3-propanediol by Citrobacter amalonaticus Y19 / Appl. Microbiol. Biotechnol 97:5001~5011
Bozell JJ / 2010 / Technology development for the production of biobased products from biorefinery carbohydrates — the US Department of Energy’s “top 10” revisited / Green Chem 12:539~554
Dmytruk OV / 2008 / Overexpression of bacterial xylose isomerase and yeast host xylulokinase improves xylose alcoholic fermentation in the thermotolerant yeast Hansenula polymorpha / FEMS Yeast Res 8:165~173
Fu J / 2014 / NADH plays the vital role for chiral pure D -(-)-2,3-butanediol production in Bacillus subtilis under limited oxygen conditions / Biotechnol. Bioeng 111:2126~2131
Ainala SK / 2013 / Glycerol assimilation and production of 1,3-propanediol by Citrobacter amalonaticus Y19 / Appl. Microbiol. Biotechnol 97:5001~5011
Bozell JJ / 2010 / Technology development for the production of biobased products from biorefinery carbohydrates — the US Department of Energy’s “top 10” revisited / Green Chem 12:539~554
Dmytruk OV / 2008 / Overexpression of bacterial xylose isomerase and yeast host xylulokinase improves xylose alcoholic fermentation in the thermotolerant yeast Hansenula polymorpha / FEMS Yeast Res 8:165~173
Fu J / 2014 / NADH plays the vital role for chiral pure D -(-)-2,3-butanediol production in Bacillus subtilis under limited oxygen conditions / Biotechnol. Bioeng 111:2126~2131
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