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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제29권 제2호
- 발행연도
- 2019.1
- 수록면
- 244 - 255 (12page)
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Xylose isomerase (XI; E.C. 5.3.1.5) catalyzes the isomerization of xylose to xylulose, which can be used to produce bioethanol through fermentation. Therefore, XI has recently gained attention as a key catalyst in the bioenergy industry. Here, we identified, purified, and characterized a XI (PbXI) from the psychrophilic soil microorganism, Paenibacillus sp. R4. Surprisingly, activity assay results showed that PbXI is not a cold-active enzyme, but displays optimal activity at 60°C. We solved the crystal structure of PbXI at 1.94-Å resolution to investigate the origin of its thermostability. The PbXI structure shows a (β/α)8-barrel fold with tight tetrameric interactions and it has three divalent metal ions (CaI, CaII, and CaIII). Two metal ions (CaI and CaII) located in the active site are known to be involved in the enzymatic reaction. The third metal ion (CaIII), located near the β4-α6 loop region, was newly identified and is thought to be important for the stability of PbXI. Compared with previously determined thermostable and mesophilic XI structures, the β1-α2 loop structures near the substrate binding pocket of PbXI were remarkably different. Site-directed mutagenesis studies suggested that the flexible β1-α2 loop region is essential for PbXI activity. Our findings provide valuable insights that can be applied in protein engineering to generate lowtemperature purpose-specific XI enzymes.
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참고문헌 (42)
참고문헌 신청
Mosier N / 2005 / Features of promising technologies for pretreatment of lignocellulosic biomass / Bioresour. Technol 96:673~686
Chiang L-C / 1981 / D-Xylulose fermentation to ethanol by Saccharomyces cerevisiae / Appl. Environ. Microbiol 42:284~289
Chandrakant P / 2000 / Simultaneous bioconversion of glucose and xylose to ethanol by Saccharomyces cerevisiae in the presence of xylose isomerase / Appl. Microbiol. Biotechnol 53:301~309
Gong C-S / 1981 / Production of ethanol from D-xylose by using D-xylose isomerase and yeasts / Appl. Environ. Microbiol 41:430~436
Zhou H / 2012 / Xylose isomerase overexpression along with engineering of the pentose phosphate pathway and evolutionary engineering enable rapid xylose utilization and ethanol production by Saccharomyces cerevisiae / Metabol. Eng 14:611~622
Chiang L-C / 1981 / D-Xylulose fermentation to ethanol by Saccharomyces cerevisiae / Appl. Environ. Microbiol 42:284~289
Chandrakant P / 2000 / Simultaneous bioconversion of glucose and xylose to ethanol by Saccharomyces cerevisiae in the presence of xylose isomerase / Appl. Microbiol. Biotechnol 53:301~309
Gong C-S / 1981 / Production of ethanol from D-xylose by using D-xylose isomerase and yeasts / Appl. Environ. Microbiol 41:430~436
Zhou H / 2012 / Xylose isomerase overexpression along with engineering of the pentose phosphate pathway and evolutionary engineering enable rapid xylose utilization and ethanol production by Saccharomyces cerevisiae / Metabol. Eng 14:611~622
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