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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제26권 제1호
- 발행연도
- 2016.1
- 수록면
- 139 - 144 (6page)
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초록· 키워드
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Synthetic oscillators are gene circuits in which the protein expression will change over time. The delay of transcription, translation, and protein folding is used to form this kind of behavior. Here, we tried to design a synthetic oscillator by a negative feedback combined with a positive feedback. With the mutant promoter PLacC repressed by LacIq and PLux activated by AHL-bound LuxR, two gene circuits, Os-LAA and Os-ASV, were constructed and introduced into LacI-deleted E. coli DH5α cells. When glucose was used as the carbon source, a low level of fluorescence was detected in the culture, and the bacteria with Os-ASV showed no oscillation, whereas a small portion of those carrying Os-LAA demonstrated oscillation behavior with a period of about 68.3 ± 20 min. When glycerol was used as the carbon source, bacteria with Os-ASV demonstrated high fluorescence value and oscillation behavior with the period of about 121 ± 21 min.
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참고문헌 (21)
참고문헌 신청
Andersen JB / 1998 / New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria / Appl. Environ. Microbiol 64:2240~2246
Andrianantoandro E / 2006 / Synthetic biology: new engineering rules for an emerging discipline / Mol. Syst. Biol 2:1~14
Becskei A / 2000 / Engineering stability in gene networks by autoregulation / Nature 405:590~593
Chen L / 2010 / Modeling Biomolecular Networks in Cells: Structure and Dynamics / Springer :217~232
Danino T / 2010 / A synchronized quorum of genetic clocks / Nature 463:326~330
Andrianantoandro E / 2006 / Synthetic biology: new engineering rules for an emerging discipline / Mol. Syst. Biol 2:1~14
Becskei A / 2000 / Engineering stability in gene networks by autoregulation / Nature 405:590~593
Chen L / 2010 / Modeling Biomolecular Networks in Cells: Structure and Dynamics / Springer :217~232
Danino T / 2010 / A synchronized quorum of genetic clocks / Nature 463:326~330
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