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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제9호
- 발행연도
- 2015.1
- 수록면
- 1,442 - 1,448 (7page)
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The flavonoid apigenin and its O-methyl derivative, genkwanin, have various biological activities and can be sourced from some vegetables and fruits. Microorganisms are an alternative for the synthesis of flavonoids. Here, to synthesize genkwanin from tyrosine, we first synthesized apigenin from p-coumaric acid using four genes (4CL, CHS, CHI, and FNS) in Escherichia coli. After optimization of different combinations of constructs, the yield of apigenin was increased from 13 mg/l to 30 mg/l. By introducing two additional genes (TAL and POMT7) into an apigenin-producing E. coli strain, we were able to synthesize 7-O-methyl apigenin (genkwanin) from tyrosine. In addition, the tyrosine content in E. coli was modulated by overexpressing aroG and tyrA. The engineered E. coli strain synthesized approximately 41 mg/l genkwanin.
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참고문헌 (25)
참고문헌 신청
Balunas MJ / 2005 / Drug discovery from medicinal plants / Life Sci 78 : 431 ~ 441
Bate-Smith EC / 2008 / The phenolic constituents of plants and their taxonomic significance / J. Linn. Soc. (Bot.) 58 : 95 ~ 173
Bong Gyu Kim / 2007 / Anthocyanin Content in Rice Is Related to Expression Levels of Anthocyanin Biosynthetic Genes / Journal of Plant Biology(한국식물학회지) 50 (2) : 156 ~
Bong-Gyu Kim / 2012 / Analysis of Flavonoid Contents and Expression of Flavonoid Biosynthetic Genes in Populus euramericana Guinier in Response to Abiotic Stress / Applied B
Brozic P / 2009 / Flavonoids and cinnamic acid derivatives as inhibitors of 17beta-hydroxysteroid dehydrogenase type 1 / Mol. Cell. Endocrinol 301 : 229 ~ 334
Bate-Smith EC / 2008 / The phenolic constituents of plants and their taxonomic significance / J. Linn. Soc. (Bot.) 58 : 95 ~ 173
Bong Gyu Kim / 2007 / Anthocyanin Content in Rice Is Related to Expression Levels of Anthocyanin Biosynthetic Genes / Journal of Plant Biology(한국식물학회지) 50 (2) : 156 ~
Bong-Gyu Kim / 2012 / Analysis of Flavonoid Contents and Expression of Flavonoid Biosynthetic Genes in Populus euramericana Guinier in Response to Abiotic Stress / Applied B
Brozic P / 2009 / Flavonoids and cinnamic acid derivatives as inhibitors of 17beta-hydroxysteroid dehydrogenase type 1 / Mol. Cell. Endocrinol 301 : 229 ~ 334
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