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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제29권 제6호
- 발행연도
- 2019.1
- 수록면
- 839 - 844 (6page)
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Anthranilate derivatives have been used as flavoring and fragrant agents for a long time. Recently, these compounds are gaining attention due to new biological functions including antinociceptive and analgesic activities. Three anthranilate derivatives, N-methylanthranilate, methyl anthranilate, and methyl N-methylanthranilate were synthesized using metabolically engineered stains of Escherichia coli. NMT encoding N-methyltransferase from Ruta graveolens, AMAT encoding anthraniloyl-coenzyme A (CoA):methanol acyltransferase from Vitis labrusca, and pqsA encoding anthranilate coenzyme A ligase from Pseudomonas aeruginosa were cloned and E. coli strains harboring these genes were used to synthesize the three desired compounds. E. coli mutants (metJ, trpD, tyrR mutants), which provide more anthranilate and/or S-adenosyl methionine, were used to increase the production of the synthesized compounds. MS/MS analysis was used to determine the structure of the products. Approximately, 185.3 μM N-methylanthranilate and 95.2 μM methyl N-methylanthranilate were synthesized. This is the first report about the synthesis of anthranilate derivatives in E. coli.
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참고문헌 (23)
참고문헌 신청
Balderas-Hernández VE / 2009 / Metabolic engineering for improving anthranilate synthesis from glucose in Escherichia coli / Microb. Cell Fact 8:19
Radwanski ER / 1995 / Tryptophan biosynthesis and metabolism : biochemical and molecular genetics / Plant Cell 7:921~934
Kutchan TM / 1995 / Alkaloid biosynthesis-The basis for metabolic engineering of medicinal plants / Plant Cell 7:1059~1070
Pillet J / 2017 / Identification of a methyltransferase catalyzing the final step of methyl anthranilate synthesis in cultivated strawberry / BMC Plant Biol 17:147
Radulović NS / 2011 / Identification of a new antinociceptive alkaloid N-methylantranilate from the essential oil of Choisya ternate Kunth / J. Ethnopharmacol 135:610~619
Radwanski ER / 1995 / Tryptophan biosynthesis and metabolism : biochemical and molecular genetics / Plant Cell 7:921~934
Kutchan TM / 1995 / Alkaloid biosynthesis-The basis for metabolic engineering of medicinal plants / Plant Cell 7:1059~1070
Pillet J / 2017 / Identification of a methyltransferase catalyzing the final step of methyl anthranilate synthesis in cultivated strawberry / BMC Plant Biol 17:147
Radulović NS / 2011 / Identification of a new antinociceptive alkaloid N-methylantranilate from the essential oil of Choisya ternate Kunth / J. Ethnopharmacol 135:610~619
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