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논문 기본 정보
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- 학술저널
- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제19권 제12호
- 발행연도
- 2009.1
- 수록면
- 1,620 - 1,627 (8page)
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The design and expression of an antihypertensive peptide
multimer (AHPM), a common precursor of 11 kinds of
antihypertensive peptides (AHPs) tandemly linked up
according to the restriction sites of gastrointestinal proteases,
were explored. The DNA fragment encoding the AHPM
was chemically synthesized and cloned into expression vector
pGEX-3X. After an optimum induction with IPTG, the
recombinant AHPM fused with glutathione S-transferase
(GST-AHPM) was expressed mostly as inclusion body in
Escherichia coli BL21 and reached the maximal production,
35% of total intracellular protein. The inclusion body
was washed, dissolved, and purified by cation-exchange
chromatography under denaturing conditions, followed
by refolding together with size-exclusion chromatography
and gradual dialysis. The resulting yield of the soluble GSTAHPM
(34 kDa) with a purity of 95% reached 399 mg/l
culture. The release of high active fragments from the
AHPM was confirmed by the simulated gastrointestinal
digestion. The results suggest that the design strategy and
production method of the AHPM will be useful to obtain a
large quantity of recombinant AHPs at a low cost.
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참고문헌 (23)
참고문헌 신청
Alting, A. C. / 1997 / Incomplete elimination of the ABBOS epitope of bovine serum albumin under simulated gastrointestinal conditions of infants / Diabetes Care 20 : 875 ~ 8
Bettadapura, J. / 1998 / Expression, purification, and encephalitogenicity of recombinant human myelin oligodendrocyte glycoprotein / J. Neurochem 70 : 1593 ~ 1599
Bradford, M. M. / 1976 / A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem
Byun, H. G. / 2001 / Purification and characterization of angiotensin I converting enzyme (ACE) inhibitory peptides from Alaska pollack (Theragra chalcogramma) skin / Process
Chen, G. W. / 2007 / Purification of angiotensin I-converting enzyme inhibitory peptides and antihypertensive effect of milk produced by protease-facilitated lactic fermentat
Bettadapura, J. / 1998 / Expression, purification, and encephalitogenicity of recombinant human myelin oligodendrocyte glycoprotein / J. Neurochem 70 : 1593 ~ 1599
Bradford, M. M. / 1976 / A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem
Byun, H. G. / 2001 / Purification and characterization of angiotensin I converting enzyme (ACE) inhibitory peptides from Alaska pollack (Theragra chalcogramma) skin / Process
Chen, G. W. / 2007 / Purification of angiotensin I-converting enzyme inhibitory peptides and antihypertensive effect of milk produced by protease-facilitated lactic fermentat
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