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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제15권 제3호
- 발행연도
- 2005.1
- 수록면
- 652 - 655 (4page)
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This paper describes the development of anenzymatic assay system for the identification of inhibitors ofisocitrate lyase (ICL), one of the key enzymes of the glyoxylatecycle that is considered as a new target for antifungal drugs. A1.6 kb DNA fragment encoding the isocitrate lyase fromCandida albicans ATCC10231 was amplified by PCR, clonedinto a vector providing His-Patch-thioredoxin-tag at the Nterminus,expressed in Escherichia coli, and purified by metalchelate affinity chromatography. The molecular mass of thepurified ICL was approximately 62 kDa, as determined bySDS-PAGE, and the enzyme activity was directly proportionalto incubation time and enzyme concentration. The effectsof itaconate-related compounds on ICL activity were alsoinvestigated. Among them, itaconic acid, 3-nitropropionate,and oxalate had strong inhibitory activities with IC50 values of5.8, 5.4 and 8.6 μg/ml, respectively. These inhibitors alsoexhibited antifungal activity on YPD agar media containingacetate as a sole carbon source, albeit at high concentration.The results indicate that the C. albicans ICL may be aregulatory enzyme playing a crucial role in fungal growth andis a prime target for antifungal agents.
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참고문헌 (15)
참고문헌 신청
A / 1999 / Honer Zu Bentrup Characterization of activity and expression ofisocitrate in Mycobacterium avium and Mycobacteriumtuberculosis 181 : 7161 ~ 7167
Bishai,W / 2000 / Microbiology:Lipid lunch for persistentpathogen 406
Bradford / 1976 / A rapid and sensitive method for thequantification of microgram quantities of protein utilizingthe principle of protein-dye binding 72 : 248 ~ 254
Britton / 2000 / The crystal structure and active site location ofisocitrate lyase from the fungus Aspergillus nidulans weeradechapon : 349 ~ 362
Chiou / 2000 / New drugsand novel targets for treatment of invasive fungal infectionsin patients with cancer 5 : 120 ~ 5 135
Bishai,W / 2000 / Microbiology:Lipid lunch for persistentpathogen 406
Bradford / 1976 / A rapid and sensitive method for thequantification of microgram quantities of protein utilizingthe principle of protein-dye binding 72 : 248 ~ 254
Britton / 2000 / The crystal structure and active site location ofisocitrate lyase from the fungus Aspergillus nidulans weeradechapon : 349 ~ 362
Chiou / 2000 / New drugsand novel targets for treatment of invasive fungal infectionsin patients with cancer 5 : 120 ~ 5 135
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