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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제19권 제3호
- 발행연도
- 2009.1
- 수록면
- 323 - 330 (8page)
이용수
초록· 키워드
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Nontuberculous mycobacteria (NTM) are a major cause
of opportunistic infections in immunocompromised patients,
making the reliable and rapid identification of NTM to
the species level very important for the treatment of such
patients. Therefore, this study evaluated the usefulness
of the novel target genes tuf and tmRNA for the
identification of NTM to the species level, using a PCRrestriction
fragment length polymorphism analysis (PRA).
A total of 44 reference strains and 17 clinical isolates of
the genus Mycobacterium were used. The 741 bp or 744 bp
tuf genes were amplified, restricted with two restriction
enzymes (HaeIII/MboI), and sequenced. The tuf gene-PRA
patterns were compared with those for the tmRNA
(AvaII), hsp65 (HaeIII/HphI), rpoB (MspI/HaeIII), and 16S
rRNA (HaeIII) genes. For the reference strains, the tuf
gene-PRA yielded 43 HaeIII patterns, of which 35 (81.4%)
showed unique patterns on the species level, whereas the
tmRNA, hsp65, rpoB, and 16S rRNA-PRAs only showed
10 (23.3%), 32 (74.4%), 19 (44.2%), and 3 (7%) unique
patterns after single digestion, respectively. The tuf gene-
PRA produced a clear distinction between closely related
NTM species, such as M. abscessus (557-84-58) and M.
chelonae (477-84-80-58), and M. kansasii (141-136-80-63-
58-54-51) and M. gastri (141-136-117-80-58-51). No difference
was observed between the tuf-PRA patterns for the reference
strains and clinical isolates. Thus, a diagnostic algorithm
using a tuf gene-targeting PRA is a promising tool with
more advantages than the previously used hsp65, rpoB,
and 16S rRNA genes for the identification of NTM to the
species level.
목차
등록된 정보가 없습니다.
참고문헌 (21)
참고문헌 신청
Adekambi, T. / 2004 / Dissection of phylogenetic relationships among 19 rapidly growing Mycobacterium species by 16S rRNA, hsp65, sodA, recA, and rpoB gene sequencing / . J.
Adekambi, T. / 2006 / rpoB gene sequence-based characterization of emerging nontuberculous mycobacteria with descriptions of Mycobacterium bolletii sp. nov., Mycobacterium ph
Afghani, B. / 1996 / Polymerase chain reaction for diagnosis of M. tuberculosis: Comparison of simple boiling and a conventional method for DNA extraction / Biochem. Mol. Med
Barends, S. / 2001 / Simultaneous and functional binding of SmpB and EF-Tu-GTP to the alanyl acceptor arm of tmRNA / J. Mol. Biol 314 : 9 ~ 21
Brown-Elliott, B. A. / 2002 / Clinical and taxonomic status of pathogenic nonpigmented or late-pigmenting rapidly growing mycobacteria / Clin. Microbiol. Rev 15 : 716 ~ 746
Adekambi, T. / 2006 / rpoB gene sequence-based characterization of emerging nontuberculous mycobacteria with descriptions of Mycobacterium bolletii sp. nov., Mycobacterium ph
Afghani, B. / 1996 / Polymerase chain reaction for diagnosis of M. tuberculosis: Comparison of simple boiling and a conventional method for DNA extraction / Biochem. Mol. Med
Barends, S. / 2001 / Simultaneous and functional binding of SmpB and EF-Tu-GTP to the alanyl acceptor arm of tmRNA / J. Mol. Biol 314 : 9 ~ 21
Brown-Elliott, B. A. / 2002 / Clinical and taxonomic status of pathogenic nonpigmented or late-pigmenting rapidly growing mycobacteria / Clin. Microbiol. Rev 15 : 716 ~ 746
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