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논문 기본 정보
- 자료유형
- 학술저널
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제7호
- 발행연도
- 2008.1
- 수록면
- 1,207 - 1,215 (9page)
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To investigate the occurrence and species diversity of
mycobacteria in waters, surface water samples were collected
monthly from the Han River and tap water samples at the
terminal sites of the distribution system. Mycobacteria in
each water sample were isolated by decontamination
using cetylpyridinium chloride (CPC) and cultivation on
Middlebrook 7H10 agar, and then identified by polymerase
chain reaction-restriction fragment length polymorphism
analysis (PRA) and sequencing of the 65-kDa heat-shock
protein gene (hsp65 gene). Mycobacteria were detected in
59% of the surface water samples and 26% of the tap
water samples. Over half of the 158 isolates could not be
identified by hsp65 PRA and gene sequencing, and several
identification discrepancies were observed between the
two methods. The most frequently isolated species was
Mycobacterium gordonae in surface water and M. lentiflavum
in tap water. M. avium complex (MAC), the most important
pathogen among environmental mycobacteria, was detected
in the surface water samples but not found in the tap
water samples. The result demonstrated that water is an
important environmental source of mycobacteria and the
combined application of hsp65 PRA and sequencing was
more reliable than hsp65 PRA alone to accurately identify
mycobacteria present in water.
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참고문헌 (20)
참고문헌 신청
AHN, JAE-HYUNG / 2006 / Analysis of Bacterial Diversity and Community Structure in Forest Soils Contaminated with Fuel Hydrocarbon / Journal of Microbiology and Biotechnology
APHA / 1998 / Standard Methods for the Examination of Water and Wastewater / American Public Health Association
BAEK, KYUNG-HWA / 2007 / Monitoring of Microbial Diversity and Activity During Bioremediation of Crude Oil-Contaminated Soil with Different Treatments / Journal of Microbiolo
Bland, C. S. / 2005 / Mycobacterial ecology of the Rio Grande. Appl. Environ / Microbiol 71 : 5719 ~ 5727
Brunello, F. / 2001 / Identification of 54 mycobacteria species by PCR-restriction fragment length polymorphism analysis of the hsp65 gene. J. Clin / Microbiol 39 : 2799 ~ 28
APHA / 1998 / Standard Methods for the Examination of Water and Wastewater / American Public Health Association
BAEK, KYUNG-HWA / 2007 / Monitoring of Microbial Diversity and Activity During Bioremediation of Crude Oil-Contaminated Soil with Different Treatments / Journal of Microbiolo
Bland, C. S. / 2005 / Mycobacterial ecology of the Rio Grande. Appl. Environ / Microbiol 71 : 5719 ~ 5727
Brunello, F. / 2001 / Identification of 54 mycobacteria species by PCR-restriction fragment length polymorphism analysis of the hsp65 gene. J. Clin / Microbiol 39 : 2799 ~ 28
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