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자료유형
학술저널
저자정보
Kim, Yang-Su (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University) Li, Ming-Shun (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University) Kang, Joong-Nam (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University) Wang, Yong (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University) Choi, Hee-Kyu (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University) Choi, Jae-Young (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University) Je, Yeon-Ho (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University)
저널정보
한국응용곤충학회 Journal of Asia-Pacific Entomology Journal of asia-pacific entomology 제8권 제3호
발행연도
2005.1
수록면
239 - 242 (4page)

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To identify novel cry1-type crystal protein genes, 100 Bacillus thuringiensis (Bt) isolates were selected on the basis of their toxicity against lepidopteran insect larvae. For rapid search of a large quantity of novel genes simultaneously, Bt isolates were randomly divided into 2 groups including 50 isolates each. About 2.4 kb PCR fragments were amplified from each group using universal oligonucleotide primers, ATG1-F and N400-R, designed to probe the toxic fragment regions of all known and possible cry1-type genes. Restriction fragment length polymorphism (RFLP) analysis revealed 3 distinct patterns in each group. One of them, A32 clone showed 87% and 91% of nucleotide sequence and deduced amino acid sequence similarities with the cry1Ea gene. Interestingly, nucleotide sequence analysis of A32 cry gene suggested that this gene might have resulted from nucleotide rearrangement between cry1Ac and cry1Ea genes.

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