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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제2호
- 발행연도
- 2008.1
- 수록면
- 334 - 337 (4page)
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초록· 키워드
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A tightly regulated gene expression system composed of a
single-copy target gene under the control of a lac promoter
derivative and lacI gene in a multicopy plasmid is proposed,
and its ability to control the flux of a metabolic pathway is
demonstrated. A model system to control the flux of acetyl-
CoA to acetyl phosphate was constructed by integrating
pta, a gene encoding phosphotransacetylase, under a tac
promoter into the chromosome of E. coli with a pta-negative
background and transforming a multicopy plasmid
containing the lacIQ gene into the strain. The production
rate of acetate was shown to be tightly controlled when
varying the concentration of the inducer (IPTG) in the
model system.
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참고문헌 (21)
참고문헌 신청
ASENJO, J. A / 2007 / A Discrete Mathematical Model Applied to Genetic Regulation and Metabolic Networks / Journal of Microbiology and Biotechnology 17 (3) : 496 ~ 510
Baneyx, F. / 1999 / Recombinant protein expression in Escherichia coli. Curr. Opin / Biotechnol 10 : 411 ~ 421
Bowers, L. M. / 2004 / Bacterial expression system with tightly regulated gene expression and plasmid copy number / Gene 340 : 11 ~ 18
Bulter, T. / 2004 / Design of artificial cell-cell communication using gene and metabolic network. Proc. Natl. Acad / Sci 101 : 2299 ~ 2304
Farmer, W. R. / 1997 / Reduction of aerobic acetate production by Escherichia coli. Appl. Environ / Microbiol 63 : 3205 ~ 3210
Baneyx, F. / 1999 / Recombinant protein expression in Escherichia coli. Curr. Opin / Biotechnol 10 : 411 ~ 421
Bowers, L. M. / 2004 / Bacterial expression system with tightly regulated gene expression and plasmid copy number / Gene 340 : 11 ~ 18
Bulter, T. / 2004 / Design of artificial cell-cell communication using gene and metabolic network. Proc. Natl. Acad / Sci 101 : 2299 ~ 2304
Farmer, W. R. / 1997 / Reduction of aerobic acetate production by Escherichia coli. Appl. Environ / Microbiol 63 : 3205 ~ 3210
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