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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제1호
- 발행연도
- 2010.1
- 수록면
- 146 - 152 (7page)
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초록· 키워드
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Streptomyces clavuligerus NRRL3585 produces a clinically important β-lactamase inhibitor, clavulanic acid (CA). In order to increase the production of CA, the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene (gap) was deleted in S. clavuligerus NRRL3585 to overcome the limited glyceraldehyde-3-phosphate pool; the replicative and integrative expressions of ccaR (specific regulator of the CA biosynthetic operon) and claR (Lys-type transcriptional activator) genes were transformed together into a deletion mutant to improve clavulanic acid production. We constructed two recombinant plasmids to enhance the production of CA in the gap1 deletion mutant of S. clavuligerus NRRL3585: pHN11 was constructed for overexpression of ccaR-claR, whereas pHN12 was constructed for their chromosomal integration. Both pHN11 and pHN12transformants enhanced the production of CA by 2.59-fold and 5.85-fold, respectively, compared with the gap1deletion mutant. For further enhancement of CA, we fed the pHN11 and pHN12 transformants ornithine and glycerol.
Compared with the gap1 deletion mutant, ornithine increased CA production by 3.24- and 6.51-fold in the pHN11 and pHN12 transformants, respectively, glycerol increased CA by 2.96- and 6.21-fold, respectively, and ornithine and glycerol together increased CA by 3.72- and 7.02-fold,respectively.
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참고문헌 (22)
참고문헌 신청
Alexander, D. C / 1998 / Investigation of the Streptomyces clavuligerus cephamycin C gene cluster and its regulation by the CcaR protein / J. Bacteriol 180 : 4068 ~ 4079
Aoki, H / 1976 / Nocardicin A, a new monocyclic β-lactam antibiotic. I. Discovery, isolation and characterization / J. Antibiot 29 : 492 ~ 500
Arias, P / 1999 / Characterization of the pathway-specific positive transcriptional regulator for actinorhodin biosynthesis in Streptomyces coelicolor A3(2) as a DNA-binding
Baggaley, K. H / 1997 / Chemistry and biosynthesis of clavulanic acid and other clavams / Nat. Prod. Rep 14 : 309 ~ 333
Bierman, M / 1992 / Plasmid cloning vectors for the conjugal transfer of DNA from E. coli to Streptomyces spp / Gene 166 : 43 ~ 49
Aoki, H / 1976 / Nocardicin A, a new monocyclic β-lactam antibiotic. I. Discovery, isolation and characterization / J. Antibiot 29 : 492 ~ 500
Arias, P / 1999 / Characterization of the pathway-specific positive transcriptional regulator for actinorhodin biosynthesis in Streptomyces coelicolor A3(2) as a DNA-binding
Baggaley, K. H / 1997 / Chemistry and biosynthesis of clavulanic acid and other clavams / Nat. Prod. Rep 14 : 309 ~ 333
Bierman, M / 1992 / Plasmid cloning vectors for the conjugal transfer of DNA from E. coli to Streptomyces spp / Gene 166 : 43 ~ 49
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