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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제17권 제8호
- 발행연도
- 2007.1
- 수록면
- 1,308 - 1,315 (8page)
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Lipase B from Candida antarctica (CalB) displayedon the cell surface of H. polymorpha has ben functionallyimproved for catalytic activity by molecular evolution. CalBmotif (CwpF). A library of CalB mutants was constructed byin vivo recombination in H. polymorpha. Several mutantswith increased whole-cel CalB activity were acquired fromscreening seven thousand transformants. The two independentmutants CalB10 and CalB14 showed an approximately 5times greater whole-cel activity than the wild-type. Whenthese mutants were made as a soluble form, CalB10 showed 6times greater activity and CalB14 showed an 11 times greateractivity compared with the wild-type. Sequence analyses ofmutant CALB genes revealed amino acid substitutions ofLeu278Pro in CalB10 and Leu278Pro/Leu219Gln in CalB14. Thesubstituted Pro278 in both mutants was located near the prolinesite of the α10 helix. This mutation was assumed to induce aconformational change in the α10 helix and increased the kcatvalue of mutant CalB approximately 6 times. Site-directed219Gln) was secreted into the culturesupernatant at an amount of approximately 3 times more withoutan increase in the CalB transcript level, compared with thewild-type.
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참고문헌 (24)
참고문헌 신청
Agaphonov, M. O / 1999 / Vectors forrapid selection of integrants with diferent plasmid copynumbers in the yeast Hansenula polymorpha DL1 15 : 541 ~ 551
Anderson / 181-204. / The use of Candida antarcticaB-lipase in organic synthesis. Biocatal. Biotransform. 16
G / / and A. W. Strasser.1991. Heterologous gene expression in Hansenula polymorpha Efficient secretion of glucoamylase. Biotechnology : 291 ~ 295
Hoegh, I / 1995 / Two lipases from Candida antarctica: Cloning and expressionin Aspergillus oryzae 73 : S869 ~ S875
Hollenberg, C. P. / 1997 / Production ofrecombinant proteins by methylotrophic yeasts 8 : 554 ~ 560
Anderson / 181-204. / The use of Candida antarcticaB-lipase in organic synthesis. Biocatal. Biotransform. 16
G / / and A. W. Strasser.1991. Heterologous gene expression in Hansenula polymorpha Efficient secretion of glucoamylase. Biotechnology : 291 ~ 295
Hoegh, I / 1995 / Two lipases from Candida antarctica: Cloning and expressionin Aspergillus oryzae 73 : S869 ~ S875
Hollenberg, C. P. / 1997 / Production ofrecombinant proteins by methylotrophic yeasts 8 : 554 ~ 560
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