지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.1
- 수록면
- 205 - 215 (11page)
이용수
초록· 키워드
오류제보하기
Chronic and heavy alcohol consumption is one of the causes of heart diseases. However, the effects of ethanol on insulin sensitivity in myocardium has been unclear. To investigate the effects of ethanol on the expression of AMP-activated protein kinase (AMPK), myocyte enhancer factor 2 (MEF2) and glucose transporter 4 (GLUT4), all of which are involved in the regulation of insulin sensitivity, in the myocardium, we performed three parts of experiments in vivo and in vitro.
I: Rats were injected with 5-amino-4-imidazolecarboxamide ribonucleotide (AICAR, 0.8 mg.kg-1) for 2 h. II:Rats received different dose (0.5, 2.5 or 5 g.kg-1.d-1) of ethanol for 22-week. III: Primary neonatal rat cardiomyocytes were isolated and treated with or without 100 mM ethanol or 1 mM AICAR for 4 h. The cardiac protein and mRNA expression of AMPKα subunits, MEF2and GLUT4 were observed by western-blotting and RT-PCR, respectively. Serum TNFα levels were assessed by ELISA. The results showed chronic ethanol exposure induced insulin resistance. Ethanol decreased the mRNA levels of AMPKα1 and α2, the protein levels of total- and phospho-AMPKα in cardiomyocytes.
Similarly, ethanol showed inhibitory effects on both the mRNA and protein levels of MEF2A and 2D,and GLUT4 in a dose-response-like fashion. Correlation analysis implied an association between phospho-AMPKα and MEF2A or MEF2D, and between the levels of MEF2 protein and GLUT4 transcription. In addition,ethanol elevated serum TNFα level. Taken together,chronic ethanol exposure decreases the expression of AMPKα and MEF2, and is associated with GLUT4 decline in rat myocardium.
목차
등록된 정보가 없습니다.
참고문헌 (20)
참고문헌 신청
Bolster DR / 2002 / AMP-activated protein kinase suppresses protein synthesis in rat skeletal muscle through down-regulated mammalian target of rapamycin (mTOR) signaling / J
Feng L / 2008 / Long-term moderate ethanol consumption restores insulin sensitivity in high-fat-fed rats by increasing GLUT4 in the adipose tissue by AMPK activation / J Endo
Fischer Y / 1997 / Insulin-induced recruitment of glucose transporter 4 (GLUT4) and GLUT1 in isolated rat cardiac myocytes / J Biol Chem 272 : 7085 ~ 7092
Fowman DT / 1988 / The effect of ethanol and its metabolites on carbohydrate, protein and lipid metabolism / Ann Clin Lab Sci 8 : 181 ~ 189
Fujii N / 2005 / AMP-activated protein kinase α2 activity is not essential for contraction-and hyperosmolarity-induced glucose transport in skeletal muscle / J Biol Chem 280
Feng L / 2008 / Long-term moderate ethanol consumption restores insulin sensitivity in high-fat-fed rats by increasing GLUT4 in the adipose tissue by AMPK activation / J Endo
Fischer Y / 1997 / Insulin-induced recruitment of glucose transporter 4 (GLUT4) and GLUT1 in isolated rat cardiac myocytes / J Biol Chem 272 : 7085 ~ 7092
Fowman DT / 1988 / The effect of ethanol and its metabolites on carbohydrate, protein and lipid metabolism / Ann Clin Lab Sci 8 : 181 ~ 189
Fujii N / 2005 / AMP-activated protein kinase α2 activity is not essential for contraction-and hyperosmolarity-induced glucose transport in skeletal muscle / J Biol Chem 280
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Aging impairs insulin-stimulated glucose uptake in rat skeletalmuscle via suppressing AMPKα
Experimental and Molecular Medicine
2007 .01
-
AMPK activators: mechanisms of action and physiological activities
Experimental and Molecular Medicine
2016 .01
-
Regulation and function of AMPK in physiology and diseases
Experimental and Molecular Medicine
2016 .01
-
C2C12 근관세포에서 상엽에 의한 AMPK의 불활성화와 AICAR로 유도된 근위축 억제의 연관성에 관한 연구
생명과학회지
2018 .04
-
AMPK γ is Required for Maintaining Epithelial Cell Structure and Polarity
생명과학회지
2011 .05
-
지구성 운동이 STZ-당뇨 유발 쥐의 허혈/재 관류 손상 후 심근의 당대사 관련 표현형 단백질과 HSP-60 단백질 발현에 미치는 영향
생명과학회지
2009 .05
-
1-Deoxynojirimycin Isolated from a Bacillus subtilis Stimulates Adiponectin and GLUT4 Expressions in 3T3-L1 Adipocytes
Journal of Microbiology and Biotechnology
2013 .01
-
Effects of Carnitine on the Lipid Metabolism in the Ethanol-Fed Rats
동물학회지
1989 .01
-
AMP kinase/cyclooxygenase-2 pathway regulates proliferation and apoptosis of cancer cells treated with quercetin
Experimental and Molecular Medicine
2009 .01
-
Effect of Ethanol Concentration on the Rates of Cell Growth and Ethanol Production in Zymomonas mobilis
미생물학회지
1985 .06
-
AMP-activated Kinase Regulates Adipocyte Differentiation Process in 3T3-L1 Adipocytes Treated with Selenium
생명과학회지
2009 .04
-
Sasa borealis extract exerts an antidiabetic effect via activation of the AMP - activated protein kinase
Nutrition Research and Practice
2013 .02
-
Sasa borealis extract exerts an antidiabetic effect via activation of the AMP - activated protein kinase
Nutrition Research and Practice
2013 .02
-
Effects of Taurine Supplementation on Mitochondrial Function in Chronic Ethanol Administered Rats
Journal of Community Nutrition
2005 .09
-
Ethanol 충격에 의한 Campylobacter jejuni의 Ethanol 내성
미생물학회지
1992 .10
-
에탄올을 투여한 흰쥐에서 식이 단백질 섭취 수준이 혈청중 지방및 효소활성에 미치는 영향
한국영양학회지
1992 .12
-
Response of Saccharomyces cerevisiae to Ethanol Stress Involves Actions of Protein Asr1p
Journal of Microbiology and Biotechnology
2010 .01
-
에탄올 장기 투여에 의한 쥐 심근조직의 산화적 스트레스와 생체내 황산화 효소활성의 변화
한국영양학회지
1996 .09
-
운동강도의 차이가 streptozotocin-유도 당뇨쥐의 가자미근 GRP-78과 GLUT-4 발현에 미치는 영향
생명과학회지
2005 .02
-
Identification and characterization of peroxisome proliferator response element in the mouse GLUT2 promoter
Experimental and Molecular Medicine
2005 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0