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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
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- 1 - 9 (9page)
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Our study aims to explore the effects of lentivirus-mediated microRNA-124 (miR-124) gene-modified bone marrow mesenchymal stem cell (BMSC) transplantation on the repair of spinal cord injury (SCI) in rats. BMSCs were isolated from the bone marrow of rats. The target gene miR-124 was identified using a luciferase-reporter gene assay. Seventy-two rats were selected for construction of the SCI model, and the rats were randomly divided into the blank group, sham group, SCI group, negative control (NC) group, overexpressed miR-124 group and si-PDXK group. The mRNA expression of miR-124 and the mRNA and protein expression of pyridoxal kinase (PDXK) were detected by quantitative real-time polymerase chain reaction and western blotting. The locomotor capacity of the rats was evaluated using the Basso, Beattie and Bresnahan (BBB) scale. Brdu, neuron-specific enolase (NSE), neurofilament (NF) and microtubule-associated protein 2 (MAP2) were detected using immunohistochemistry. The expression levels of thyrotropin-releasing hormone (TRH), prostacyclin (PGI2) and gangliosides (GM) were measured using an enzyme-linked immunosorbent assay. PDXK was identified as the target gene of miR-124. The overexpressed miR-124 group exhibited higher miR-124 expression than the SCI, NC and si-PDXK groups. Compared with the SCI and NC groups, the PDXK expression was downregulated in the overexpressed miR-124 and si-PDXK groups, and the BBB scores were significantly increased 7, 21 and 35 days after transplantation. The double-labeled positive cell densities (Brdu+NSE/NF/MAP2) and the expression levels of TRH, PGI2 and GM in the overexpressed miR-124 group were significantly higher than those in the NC and SCI groups. These results indicated that miR-124 targeted PDXK to accelerate the differentiation of BMSCs into neurocytes and promote SCI repair.
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참고문헌 (24)
참고문헌 신청
/ 2016 / Spinal Cord Injury (SCI) facts and figures at a glance / J Spinal Cord Med 39 : 493 ~ 494
Basso DM / 1995 / A sensitive and reliable locomotor rating scale for open field testing in rats / J Neurotrauma 12 : 1 ~ 21
Cheng LC / 2009 / miR-124 regulates adult neurogenesis in the subventricular zone stem cell niche / Nat Neurosci 12 : 399 ~ 408
Czarnecka J / 2016 / Neurogenic differentiation of mesenchymal stem cells induces alterations in extracellular nucleotides metabolism / J Cell Biochem 118 : 478 ~ 486
Furuta M / 2010 / miR-124 and miR-203 are epigenetically silenced tumor-suppressive microRNAs in hepatocellular carcinoma / Carcinogenesis 31 : 766 ~ 776
Basso DM / 1995 / A sensitive and reliable locomotor rating scale for open field testing in rats / J Neurotrauma 12 : 1 ~ 21
Cheng LC / 2009 / miR-124 regulates adult neurogenesis in the subventricular zone stem cell niche / Nat Neurosci 12 : 399 ~ 408
Czarnecka J / 2016 / Neurogenic differentiation of mesenchymal stem cells induces alterations in extracellular nucleotides metabolism / J Cell Biochem 118 : 478 ~ 486
Furuta M / 2010 / miR-124 and miR-203 are epigenetically silenced tumor-suppressive microRNAs in hepatocellular carcinoma / Carcinogenesis 31 : 766 ~ 776
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