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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.1
- 수록면
- 35 - 42 (8page)
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In order to develop an anti-human TNF-α mAb, mice were imunized with recombinant human TNF-α. A murine mAb, TSK114, which showed the highest bind-ing activity for human TNF-α was selected and characterized. TSK114 specifically bound to human TNF-α without cross-reactivity with the homologous α and human TNF-β. TSK114 was found to be of IgG1 isotype with κ light chain. The nucleotide se-quences of the variable regions of TSK114 heavy and light chains were determined and analyzed for the us-age of gene families for the variable (V), diversity (D), and joining (J) segments. Kinetic analysis of TSK114 binding to human TNF-α by surface plasmon reso-nance technique revealed a binding affinity (KDpM, which is about 1,000- and 100-fold higher than those of clinically relevant infliximab (Remicade) and adalimumab (Humira) mAbs, respectively. TSK114 neutralized human TNF-α-mediated cytotoxicity in proportion to the concentration, exhibiting about 4-fold greater eficiency than those of infliximab and adalimumab in WEHI 164 cells used as an in vitro model system. These results suggest that TSK114 has the po-α-neutr-alizing antibody with picomolar affinity.
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참고문헌 (24)
참고문헌 신청
Austgulen R / 1986 / Human monocyte cytotoxic factor mediates cytolysis of WEHI 164 cells / Cell Immunol 98 : 211 ~ 20
Balkwill FR / 1989 / The cytokine network / Immunol Today 10 : 299 ~ 304
Bazzoni F / 1996 / The tumor necrosis factor ligand and receptor families / N Engl J Med 334 : 1717 ~ 25
Benucci M / 2005 / Adalimumab (anti-TNF-alpha) therapy to improve the clinical course of adult-onset Still's disease: the first case report / Clin Exp Rheumatol 23 : 733 ~
Beutler B / 1988 / Tumor necrosis, cachexia, shock and inflammation: a common mediator / Ann Rev Biochem 57 : 505 ~ 18
Balkwill FR / 1989 / The cytokine network / Immunol Today 10 : 299 ~ 304
Bazzoni F / 1996 / The tumor necrosis factor ligand and receptor families / N Engl J Med 334 : 1717 ~ 25
Benucci M / 2005 / Adalimumab (anti-TNF-alpha) therapy to improve the clinical course of adult-onset Still's disease: the first case report / Clin Exp Rheumatol 23 : 733 ~
Beutler B / 1988 / Tumor necrosis, cachexia, shock and inflammation: a common mediator / Ann Rev Biochem 57 : 505 ~ 18
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