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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2003.1
- 수록면
- 285 - 292 (8page)
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The acrosome reaction is a Ca2+-dependent exocytotic
process that is a prerequisite step for fertilization.
External calcium entry through voltage-activated
Ca2+ channels is known to be essential in
inducing the acrosome reaction of mammalian spermatozoa.
Due to their complex geometry, however,
electrophysiological identification of sperm
Ca2+ channels has been limited. Here we identified
Ca2+ channel mRNAs expressed in motile human
sperm using RT-PCR and their levels were compared
using RNase protection assays. L-type, non-
L-type, and T-type Ca2+ channel mRNAs were detected
by RT-PCR using degenerate primers. Cloning
and sequencing of the PCR products revealed
α1B, α1C, α1E, α1G, and α1H sequences. RT-PCR
using specific prim ers repeatedly detected α1B,
α1C, α1E, α1G, and α1H mRNAs, and additionally
α1I mRNA. But α1A and α1D messages were not
detected. Relative expression levels of the detected
Ca2+ channel subtypes were compared by RNase
protection assays. The abundance of detected
mRNA messages was in the following order: α1H
≥α1G≥α1E≥α1B＞α1C＞α1I. These findings indicated
that human motile sperm express multiple
voltage-activated Ca2+ channel RNAs among which
T-type and non-L-type channel messages are likely
to be predominantly expressed. Based on their relative
expression levels, we propose that not only
T-type but also non-L-type calcium channels may
be major gates for the external calcium influx, required
for the acrosome reaction.
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