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논문 기본 정보

자료유형
학술저널
저자정보
Bae, In-Ha (Department of Biology, College of Natural Sciences, Sungshin Women&prime) Yoon, Sook-Young (s University) Yoon, Yong-Dal (Department of Biology, College of Natural Sciences, Sungshin Women&prime) Kim, Moon-Kyoo (s University) Kim, Hae-Kwon (Department of Biology, College of Natural Sciences, Hanyang University)
저널정보
한국통합생물학회 Korean journal of biological sciences Korean journal of biological sciences 제3권 제1호
발행연도
1999.1
수록면
53 - 58 (6page)

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초록· 키워드

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The immunocytochemical method was used to identify the existence of voltage-dependent $Ca^{2+}$-channels in mouse follicular oocytes. Three types of voltage-dependent $Ca^{2+}$-channels were shown to exist in the follicular oocytes for the first time, the P/Q-type $Ca^{2+}$-channel, the N-type $Ca^{2+}$-channel, and the L-type $Ca^{2+}$-channel. Among proven $Ca^{2+}$-channels distributions of the P/Q-type $Ca^{2+}$-channel and L-type $Ca^{2+}$-channel showed localized staining (clustered pattern) on the oolemma. The distribution of the P/Q-type $Ca^{2+}$-channel showed all localized staining, and the range of localized staining was from 1 to 8 in staining intensity. As the staining intensity increased from 1 to 8, the number of localized staining decreased. The L-type $Ca^{2+}$-channel are homogeneously stained (29.4%-54.2%), while some of them (around 28.7%-44.1%) showed localized staining on the oolemma. However, the rest of them showed no staining at all (17.1%- 26.5%). On the contrary, the N-type $Ca^{2+}$-channel showed mostly homogeneous staining, while nonstaining oocytes were around 33.8%. The rest showed localized staining (10%). However, staining intensity was much weaker than those of the P/Q-type and L-type $Ca^{2+}$-channel. In fact, the N-type $Ca^{2+}$-channel has been known to exist only in neurons (from ectoderm origin), but it is unknown how the N-type $Ca^{2+}$-channel exists in the follicular oocytes (from mesoderm origin). Further studies are needed to examine the expression of $Ca^{2+}$-channels during the developmental stages of the oocytes.

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