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- 2015.1
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The elucidation of the molecular mechanisms underlying the differentiation and proliferation of human adipose tissue-derived stromal cells (hADSCs) represents a critical step in the development of hADSCs-based cellular therapies. To examine the role of the microRNA-103a-3p (miR-103a-3p) in hADSCs functions, miR-103a-3p mimics were transfected into hADSCs in order to overexpress miR-103a-3p. Osteogenic differentiation was induced for 14 days in an osetogenic differentiation medium and assessed by using an Alizarin Red S stain. The regulation of the expression of CDK6 (cyclin-dependent kinase 6), a predicted target of miR-103a-3p, was determined by western blot, real-time PCR and luciferase reporter assays. Overexpression of miR-103a-3p inhibited the proliferation and osteogenic differentiation of hADSCs. In addition, it downregulated protein and mRNA levels of predicted target of miR-103a-3p (CDK6 and DICER1). In contrast, inhibition of miR-103a-3p with 2′O methyl antisense RNA increased the proliferation and osteogenic differentiation of hADSCs. The luciferase reporter activity of the construct containing the miR-103a-3p target site within the CDK6 and DICER1 3′-untranslated regions was lower in miR-103a- 3p-transfected hADSCs than in control miRNA-transfected hADSCs. RNA interference-mediated downregulation of CDK6 and DICER1 in hADSCs inhibited their proliferation and osteogenic differentiation. The results of the current study indicate that miR-103a-3p regulates the osteogenic differentiation of hADSCs and proliferation of hADSCs by direct targeting of CDK6 and DICER1 partly. These findings further elucidate the molecular mechanisms governing the differentiation and proliferation of hADSCs.
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참고문헌 (23)
참고문헌 신청
Boren T / 2008 / MicroRNAs and their target messenger RNAs associated with endometrial carcinogenesis / Gynecol Oncol 110 : 206 ~ 215
Cairo S / 2010 / Stem cell-like micro-RNA signature driven by Myc in aggressive liver cancer / Proc Natl Acad Sci USA 107 : 20471 ~ 20476
Chakraborty C / 2013 / miRNAs in insulin resistance and diabetes-associated pancreatic cancer : the 'minute and miracle' molecule moving as a monitor in the 'genomic galaxy'
Dweep H / 2011 / miRWalk?database : prediction of possible miRNA binding sites by ‘walking’ the genes of thr
Geng L / 2014 / MicroRNA-103promotes colorectal cancer by targeting tumor suppressor DICER and PTEN / Int J Mol Sci 15 : 8458 ~ 8472
Cairo S / 2010 / Stem cell-like micro-RNA signature driven by Myc in aggressive liver cancer / Proc Natl Acad Sci USA 107 : 20471 ~ 20476
Chakraborty C / 2013 / miRNAs in insulin resistance and diabetes-associated pancreatic cancer : the 'minute and miracle' molecule moving as a monitor in the 'genomic galaxy'
Dweep H / 2011 / miRWalk?database : prediction of possible miRNA binding sites by ‘walking’ the genes of thr
Geng L / 2014 / MicroRNA-103promotes colorectal cancer by targeting tumor suppressor DICER and PTEN / Int J Mol Sci 15 : 8458 ~ 8472
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