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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2007.1
- 수록면
- 499 - 507 (9page)
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Cytokine and chemokine receptors play a key role in inflammation caused by rheumatoid arthritis (RA). Two isoforms of human CC chemokine receptor R2 (CCR2), the receptor of monocyte chemoattractant protein 1 (MCP-1), have been identified but their relative expression in fibroblast-like synoviocytes (FLS) and their contribution to inflammatory responses mediated by MCP-1 or inflammatory cytokines in patients with RA remain uncertain. We examined the pattern of expression of two CCR2 isoforms upon stimulation by proinflammatory cytokines and CD40 ligation. FLS were prepared from the synovial tissues of RA patients and cultured in the presence of MCP-1, soluble CD40 ligand (sCD40L), TGF-β, IL-1β, IL-18, IL-15, and LPS.
CCR2A and CCR2B expression was examined by immunohistochemistry, RT-PCR and western blotanalysis. IL-15, TNF-α, and MCP-1 production was determined by ELISA. Immunohistochemistry showed that CCR2A is highly expressed in RA synovium compared with OA synovium. Transcripts of both CCR2A and CCR2B were detected in FLS. Exogenous MCP-1, CD40L, TGF-β, and IL-15 significantly increased the expression of CCR2A but not CCR2B. Exposure of FLS to sCD40L caused strong upregulation of CCR2A but not of CCR2B protein expression. MCP-1 increased the proliferation of FLS and the production of IL-15, TNF-α, and IL-18. Because CCR2A is the main target of regulation by cytokines and CD40 ligation, the relatively higher expression of CCR2A on the cell surface suggests that this isoform of MCP-1 receptor functions as the principal mediator of inflammatory signals in RA FLS.
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참고문헌 (24)
참고문헌 신청
[학술
Abdi R / 2002 / Chemokine receptor polymor-phism and risk of acute rejection in human renal trans-plantation 13 : 754 ~ 758
Bartoli C / 2001 / CCR2A and CCR2B, the two isoforms of the monocyte chemoattractant protein-1 receptor are up-regulated and expressed by different cell subsets in idiopathic
Brhl H / 2004 / Dual role of CCR2 during initiation and progression of collagen-induced arthritis: evidence for regulatory activity of CCR2+ T cells 172 : 890 ~ 898
Brodmerkel CM / 2005 / Discovery and pharma- cological characterization of a novel rodent-active CCR2 antagonist, INCB3344 175 : 5370 ~ 5378
Abdi R / 2002 / Chemokine receptor polymor-phism and risk of acute rejection in human renal trans-plantation 13 : 754 ~ 758
Bartoli C / 2001 / CCR2A and CCR2B, the two isoforms of the monocyte chemoattractant protein-1 receptor are up-regulated and expressed by different cell subsets in idiopathic
Brhl H / 2004 / Dual role of CCR2 during initiation and progression of collagen-induced arthritis: evidence for regulatory activity of CCR2+ T cells 172 : 890 ~ 898
Brodmerkel CM / 2005 / Discovery and pharma- cological characterization of a novel rodent-active CCR2 antagonist, INCB3344 175 : 5370 ~ 5378
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