지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 596 - 603 (8page)
이용수
초록· 키워드
오류제보하기
The homing properties of adipose tissue-derived mesenchymal stem cells (AdMSCs) have stimulated intravenous applications for their use in stem cell therapy.
However, the soluble factors and corresponding cellular receptors responsible for inducing chemotaxis of AdMSCs have not yet been reported. In the present study, the migration capacity of human AdMSCs (hAdMSCs) toward various cytokines or growth factors (GFs) and the expression of their receptors were determined. In a conventional migration assay,PDGF-AB, TGF-β1, and TNF-α showed the most effective chemoattractant activity. When AdMSCs were preincubated with various chemokines or GF, and then allowed to migrate toward medium containing 10% FBS,those preincubated with TNF-α showed the highest migratory activity. Next, hAdMSCs were either preincubated or not with TNF-α, and allowed to migrate in response to various GFs or chemokines. Prestimulation with TNF-α increased the migration activity of hAdMSCs compared to unstimulated hAdMSCs. When analyzed by FACS and RT-PCR methods, hAdMSCs were found to express C-C chemokine receptor type 1(CCR1), CCR7, C-X-C chemokine receptor type 4(CXCR4), CXCR5, CXCR6, EGF receptor, fibroblast growth factor receptor 1, TGF-β receptor 2, TNF receptor superfamily member 1A, PDGF receptor A and PDGF receptor B at both the protein and the mRNA levels. These results indicate that the migration capacity of hAdMSCs is controlled by various GFs and chemokines. Prior in vitro modulation of the homing capacity of hAdMSCs could stimulate their movement into injured sites in vivo when administered intravenously,thereby improving their therapeutic potential.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Bacou F / 2004 / Transplantation of adipose tissue-derived stromal cells increases mass and functional capacity of damaged skeletal muscle / Cell Transplant 13 : 103 ~ 111
Banas A / 2008 / IFATS collection: in vivo therapeutic potential of human adipose tissue mesenchymal stem cells after transplantation into mice with liver injury / Stem Cells
Cai L / 2009 / IFATS collection: Human adipose tissue-derived stem cells induce angiogenesis and nerve sprouting following myocardial infarction, in conjunction with potent p
Chen J / 2000 / Intracerebral transplantation of bone marrow with BDNF after MCAo in rat / Neuropharmacology 39 : 711 ~ 716
Cho KS / 2009 / IFATS COLLECTION: IMMUNOMODULATORY EFFECTS OF ADIPOSE TISSUE-DERIVED STEM CELLS IN AN ALLERGIC RHINITIS MOUSE MODEL / STEM CELLS 27 (1) : 259 ~ 265
Banas A / 2008 / IFATS collection: in vivo therapeutic potential of human adipose tissue mesenchymal stem cells after transplantation into mice with liver injury / Stem Cells
Cai L / 2009 / IFATS collection: Human adipose tissue-derived stem cells induce angiogenesis and nerve sprouting following myocardial infarction, in conjunction with potent p
Chen J / 2000 / Intracerebral transplantation of bone marrow with BDNF after MCAo in rat / Neuropharmacology 39 : 711 ~ 716
Cho KS / 2009 / IFATS COLLECTION: IMMUNOMODULATORY EFFECTS OF ADIPOSE TISSUE-DERIVED STEM CELLS IN AN ALLERGIC RHINITIS MOUSE MODEL / STEM CELLS 27 (1) : 259 ~ 265
이 논문의 저자 정보
최근 본 자료
댓글(0)
0