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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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Background: N-containing bisphosphonates (BPs), such as pamidronate and risedronate, can inhibit osteoclastic function and reduce osteoclast number by inducing apoptotic cell death in osteoclasts. The aim of this study is to demonstrate the effect of pamidronate, second generation nitrogen-containing BPs and to elucidate matrix metalloproteinases (MMPs) mRNA expression under serum starvation and/or tumor necrosis factor alpha (TNF-α) stimulation on metabolism of intervertebral disc (IVD) cells in vitro.
Methods: Firstly, to test the effect of pamidronate on IVD cells in vitro, various concentrations (10-12 , 10-10 , 10-8 , and 10-6 M) of pamidronate were administered to IVD cells. Then DNA and proteoglycan synthesis were measured and messenger RNA (mRNA) expressions of type I collagen, type II collagen, and aggrecan were analyzed. Secondly, to elucidate the expression of MMPs mRNA in human IVD cells under the lower serum status, IVD cells were cultivated in full serum or 1% serum. Thirdly, to elucidate the expression of MMPs mRNA in IVD cells under the stimulation of 1% serum and TNF-α (10 ng/mL) In this study, IVD cells were cultivated in three dimensional alginate bead. Results: Under the lower serum culture, IVD cells in alginate beads showed upregulation of MMP 2, 3, 9, 13 mRNA. The cells in lower serum and TNF-α also demonstrated upregulation of MMP2, 3, 9, and 13 mRNA. The cells with various doses of pamidronate and lower serum and TNF-α were reveled partial down-regulation of MMPs. Conclusions: Pamidronate, Ncontaining second generation BPs, was safe in metabolism of IVD in vitro maintaining chondrogenic phenotype and matrix synthesis, and down-regulated TNF-α induced MMPs expression.
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참고문헌 (40)
참고문헌 신청
Hughes DE / 1995 / Bisphosphonates promote apoptosis in murine osteoclasts in vitro and in vivo / J Bone Miner Res 10:1478~1487
Russell RG / 1999 / Bisphosphonates : from the laboratory to the clinic and back again / Bone 25:97~106
Suri S / 2001 / Nitrogen-containing bisphosphonates induce apoptosis of Caco-2 cells in vitro by inhibiting the mevalonate pathway : a model of bisphosphonate-induced gastrointestinal toxicity / Bone 29:336~343
Alakangas A / 2002 / Alendronate disturbs vesicular trafficking in osteoclasts / Calcif Tissue Int 70:40~47
Mayahara M / 2003 / Cellular mechanism of inhibition of osteoclastic resorption of bone and calcified cartilage by long-term pamidronate administration in ovariectomized mature rats / Anat Rec A Discov Mol Cell Evol Biol 274:817~826
Russell RG / 1999 / Bisphosphonates : from the laboratory to the clinic and back again / Bone 25:97~106
Suri S / 2001 / Nitrogen-containing bisphosphonates induce apoptosis of Caco-2 cells in vitro by inhibiting the mevalonate pathway : a model of bisphosphonate-induced gastrointestinal toxicity / Bone 29:336~343
Alakangas A / 2002 / Alendronate disturbs vesicular trafficking in osteoclasts / Calcif Tissue Int 70:40~47
Mayahara M / 2003 / Cellular mechanism of inhibition of osteoclastic resorption of bone and calcified cartilage by long-term pamidronate administration in ovariectomized mature rats / Anat Rec A Discov Mol Cell Evol Biol 274:817~826
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