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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.1
- 수록면
- 954 - 959 (6page)
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Purpose: The purpose of this study is to investigate the mechanism of cellular proliferation of electromagnetic field (EMF) on human intervertebral disc (IVD) cells.
Materials and Methods: Human IVD cells were cultured three-dimensionally in alginate beads. EMF was exposed to IVD cells with 650 Ϊ, 1.8 millitesla magnetic flux density, 60 Hz sinusoidal wave. Cultures were divided into a control and EMF group. Cytotoxicity, DNA synthesis and proteoglycan synthesis were measured by MTT assay, [3H]-thymidine, and [35S]-sulfate incorporation. To detect phenotypical expression, reverse transcription-polymerase chain reactions (RT-PCR) were performed for aggrecan, collagen type I, and type II mRNA expression. To assess action mechanism of EMF, IVD cells were exposed to EMF with NG-Monomethyl-L-arginine (NMMA) and acetylsalicylic acid (ASA). Results: There was no cytotoxicity in IVD cells with the EMF group in MTT assay. Cellular proliferation was observed in the EMF group (p < 0.05). There was no difference in newly synthesized proteoglycan normalized by DNA synthesis between the EMF group and the control. Cultures with EMF showed no significant change in the expression of aggrecan, type I, and type II collagen mRNA compared to the control group. Cultures with NMMA (blocker of nitric oxide) or ASA (blocker of prostaglandin E2) exposed to EMF demonstrated decreased DNA synthesis compared to control cultures without NMMA or ASA (p < 0.05). Conclusion: EMF stimulated DNA synthesis in human IVD cells while no significant effect on proteoglycan synthesis and chondrogenic phenotype expressions. DNA synthesis was partially mediated by nitric oxide and prostaglandin E2. EMF can be utilized to stimulate proliferation of IVD cells, which may provide efficient cell amplification in cell therapy to degenerative disc disease.
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참고문헌 (24)
참고문헌 신청
Borsalino G / 1988 / Electrical stimulation of human femoral intertrochanteric osteotomies. Double-blind study / Clin Orthop Relat Res 237 : 256 ~ 263
Chelberg MK / 1995 / Identification of heterogeneous cell populations in normal human intervertebral disc / J Anat 186 : 43 ~ 53
Colson DJ / 1988 / Treatment of delayed- and non-union of fractures using pulsed electromagnetic fields / J Biomed Eng 10 : 301 ~ 304
De Mattei M / 2005 / Changes in polyamines, c-myc and c-fos gene expression in osteoblast-like cells exposed to pulsed electromagnetic fields / Bioelectromagnetics 26 : 207 ~
Fitzsimmons RJ / 1994 / Growth factors and electromagnetic fields in bone / Clin Plast Surg 21 : 401 ~ 406
Chelberg MK / 1995 / Identification of heterogeneous cell populations in normal human intervertebral disc / J Anat 186 : 43 ~ 53
Colson DJ / 1988 / Treatment of delayed- and non-union of fractures using pulsed electromagnetic fields / J Biomed Eng 10 : 301 ~ 304
De Mattei M / 2005 / Changes in polyamines, c-myc and c-fos gene expression in osteoblast-like cells exposed to pulsed electromagnetic fields / Bioelectromagnetics 26 : 207 ~
Fitzsimmons RJ / 1994 / Growth factors and electromagnetic fields in bone / Clin Plast Surg 21 : 401 ~ 406
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