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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2004.1
- 수록면
- 1,121 - 1,126 (6page)
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Rapamycin, a macrocyclic lactone, is effective in reducing the incidence of acute rejection after renal transplantation. The inhibitory effects of rapamycin on lymphocyte proliferation and the molecular mechanisms that were involved have been described. However, its effects on glomerular mesangial cells have not been clearly understood, and here, we examined the effect of rapamycin on platelet-derived growth factor (PDGF)- induced extracellular matrix synthesis as well as cell proliferation in mesangial cells. Rat mesangial cells were isolated from the glomeruli of Sprague-Dawley rats and cultured with Dulbecco's modified Eagles medium containing 20% fetal bovine serum. Different concentrations of rapamycin were administered 1 hour before the addition of 10ng/ml of PDGF into growth arrested and synchronized cells. Cell proliferation was assessed by [3H]thymidine incorporation, total collagen synthesis by [3H]proline incorporation, and fibronectin secretion into the medium by Western blot analysis. In the mesangial cells, PDGF increased cell proliferation by 4.6-fold, total collagen synthesis by 1.8-fold, and fibronectin secretion by 3.2-fold. Rapamycin above 10nM significantly inhibited PDGF-induced proliferation and collagen synthesis, but the treatment of rapamycin up to 1μM did not show any significant effects on PDGF-induced fibronectin secretion. These inhibitory effects of rapamycin on PDGF-induced mesangial cell proliferation and collagen synthesis reflect the potential value of rapamycin in the prevention and treatment of glomerulosclerosis in patients with chronic allograft nephropathy.
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참고문헌 (27)
참고문헌 신청
/ 1991 / Palatelet-derived growth factor (PDGF) and PDGF receptor are induced in mesangial proliferation nephritis in the rat 88 : 6560 ~ 6564
/ 1992 / Glomerular cell proliferation and PDGF expression precede glomerulosclerosis in the remnant kidney model 41 : 297 ~ 309
/ 1992 / Inhibition of mesangial cell proliferarion and matrix expansion in glomerulonephritis in the rat by antibody to platelet-derived growth factor 175 : 1413 ~ 1416
/ 1992 / Platelet-derived growth factor: A potentially important cytokine in glomerular disease 41 : 590 ~ 594
/ 1992 / The pathology of the mesangium 41 : 524 ~ 529
/ 1992 / Glomerular cell proliferation and PDGF expression precede glomerulosclerosis in the remnant kidney model 41 : 297 ~ 309
/ 1992 / Inhibition of mesangial cell proliferarion and matrix expansion in glomerulonephritis in the rat by antibody to platelet-derived growth factor 175 : 1413 ~ 1416
/ 1992 / Platelet-derived growth factor: A potentially important cytokine in glomerular disease 41 : 590 ~ 594
/ 1992 / The pathology of the mesangium 41 : 524 ~ 529
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