지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2007.7
- 수록면
- 13 - 20 (8page)
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Purpose: Rapamycin (RPM) and its analogues are known for their potent immunosuppressant and anti-proliferative properties, which stem from their ability to modulate the signal transduction pathways involved in cell cycle progression from the G1 to S phase. Thus, RPM has been shown to inhibit the proliferation of a number of non-immune cell types, including hepatocytes, vascular smooth cells and fibroblasts. In addition to its effects on proliferation, RPM may also play a role in the regulation of apoptosis under certain circumstances.
Methods: The effects of RPM on the activation, proliferation and expression of cytotoxic effector molecules were examined on Molt-4 human T-lymphocyte by determining its effects on apoptosis, cell viability, reactive oxygen species (ROS) production and mitochondrial dysfunction. Cells were cultured in the presence or absence of RPM, and then analyzed by Flow cytometry after staining with PI (propidium iodide).
Results: The viability of Molt-4 T cells dose- and time-dependently decreased on the addition of RPM.
Conclusion: RPM induced cytotoxicity was characterized by G2/M phase cell cycle arrest. In addition, a pharmacological scavenging study of ROS, including H₂O₂, revealed the cytotoxicity was mainly induced by the generation of ROS, which might modulate the expression of Bak protein and mitochondrial dysfunction.
Methods: The effects of RPM on the activation, proliferation and expression of cytotoxic effector molecules were examined on Molt-4 human T-lymphocyte by determining its effects on apoptosis, cell viability, reactive oxygen species (ROS) production and mitochondrial dysfunction. Cells were cultured in the presence or absence of RPM, and then analyzed by Flow cytometry after staining with PI (propidium iodide).
Results: The viability of Molt-4 T cells dose- and time-dependently decreased on the addition of RPM.
Conclusion: RPM induced cytotoxicity was characterized by G2/M phase cell cycle arrest. In addition, a pharmacological scavenging study of ROS, including H₂O₂, revealed the cytotoxicity was mainly induced by the generation of ROS, which might modulate the expression of Bak protein and mitochondrial dysfunction.
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REFERENCES
참고문헌 (27)
참고문헌 신청
1. [학술지(정기간행물)] - 최윤석 / 2003 / 생체 신이식에서 daclizumab의 임상적 치료효과 / 대한내과학회지 65 (5) : 568 ~ 576
2. [학술지(정기간행물)] - Cho WH / 2004 / Retrospective study comparing cyclosporine microemulsion and tacrolimus in living donor renal transplant recipient / J Korean Soc Transplant 18 : 23 ~ 30
3. [학술지(정기간행물)] - Schmelzle T / 2000 / TOR, a central controller of cell growth / Cell Review 103 : 253 ~ 262
4. [학술지(정기간행물)] - Marx SO / 2001 / Bench to bedside: the development of rapamycin and its application to stent restenosis / Circulation 104 : 852 ~ 855
5. [학술지(정기간행물)] - Saunders RN / 2001 / Rapamycin in transplantation : a review of the evidence / Kidney Int 59 : 3 ~ 16
2. [학술지(정기간행물)] - Cho WH / 2004 / Retrospective study comparing cyclosporine microemulsion and tacrolimus in living donor renal transplant recipient / J Korean Soc Transplant 18 : 23 ~ 30
3. [학술지(정기간행물)] - Schmelzle T / 2000 / TOR, a central controller of cell growth / Cell Review 103 : 253 ~ 262
4. [학술지(정기간행물)] - Marx SO / 2001 / Bench to bedside: the development of rapamycin and its application to stent restenosis / Circulation 104 : 852 ~ 855
5. [학술지(정기간행물)] - Saunders RN / 2001 / Rapamycin in transplantation : a review of the evidence / Kidney Int 59 : 3 ~ 16
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UCI(KEPA) : I410-ECN-0101-2013-514-003555935