전정 유모세포에서 Minocycline 전 처리를 통한 Neomycin 이독성예방효과;Reactive Oxygen Species와 Caspase-3 활성억제

김영생; 안진철; 백인영; 이정구; 정재윤

추천

검색

자료유형: 학술저널

저자정보: 김영생 안진철 백인영 이정구 정재윤

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제49권 제12호

발행연도: 2006.1

수록면: 1,159 - 1,164 (6page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Background and Objectives：Aminoglycosides, a commonly used antibiotic agent, destroys the sensory hair cells in the cochlearand vestibular system leading to irreversible hearing loss and balance problem. Minocycline, a second-generation tetracyclineantibiotic, has been known to possess anti-apoptotic properties in addition to its antimicrobial action. We hypothesized thatminocycline would attenuate aminoglycosides induced vestibulotoxicity in rat utricles. Subjects and Method：Utricular maculaeprepared from postnatal day 3-4 rats were treated with neomycin alone or in combination with minocycline. For hair cell count,utricles were stained with phalloidin- FITC. Reactive oxygen species (ROS) was assessed using the fluorescent probe, hydrofluorescentdiacetate acetyl ester (H2DCFDA). Caspase-3 activity was also examined using the fluorescent caspase-3 substrate.Results：Neomycin induced dose-dependent loss of utricular hair cells. Minocycline reduced ROS production and caspase-3activation in neomycin treated utricular hair cells. Conclusion：Minocycline has protective effect in neomycin induced ototoxicityin rat utricle by inhibiting ROS production and caspase-3 activity. (Korean J Otolaryngol 2006;49:1159-64)

#미노사이클린·Caspase·활성산소·난형낭반 #Minocycline·Caspase-3·Reactive oxygen species·Utricle.

참고문헌 (19)

참고문헌 신청

/ 1997 / Reactive oxygen species inchick hair cells after gentamicin exposure in vitro 104 : 1 ~ 14

/ 1999 / Aminoglycoside antibiotics restore dystrophy function to skeletal musclesof mdx mice 104 (4) : 375 ~ 381

/ 1999 / Caspase-3-dependent cleavage of Bcl-2 promotes releaseof cytochrome c 274 (30) : 21155 ~ 21161

/ 1999 / Dynamic studiesof ototoxicity in mature avian auditory epithelium 884 : 389 ~ 409

/ 2000 / Aminoglycoside antibiotics 5 (1) : 3 ~ 22

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!