지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한이비인후과학회 Clinical and Experimental Otorhinolaryngology Clinical and Experimental Otorhinolaryngology 제6권 제4호
- 발행연도
- 2013.1
- 수록면
- 219 - 225 (7page)
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초록· 키워드
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Objectives. Trimetazidine (TMZ) is known to reduce the generation of oxygen-derived free radicals. The objective of the present study was to evaluate the effects of TMZ on neomycin-induced ototoxicity in transgenic zebrafish (Brn3C: EGFP).
Methods. Five-day, postfertilization zebrafish larvae were exposed to 125 μM neomycin and one of the following TMZ concentrations for 1 hour: 10 μM, 100 μM, 500 μM, 1,000 μM, 1,500 μM, or 2,000 μM. Hair cells within the neuromasts of the supraorbital (SO1 and SO2), otic (O1), and occipital (OC1) lateral lines were analyzed using fluorescence microscopy and confocal microscopy (n=10). Hair cell survival was calculated as a percentage of hair cells in the control group that were not exposed to neomycin. Ultrastructural changes were evaluated using scanning electron microscopy.
Results. TMZ protected against neomycin-induced hair cell loss in the neuromasts (TMZ 1,000 μM, 11.2±0.4 cells; 125 μM neomycin only, 4.2±0.5 cells; n=10; P<0.05) and decreased the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end labeling (TUNEL) reaction. In the ultrastructural analysis, structures of mitochondria and hair cells within the neuromasts were preserved in zebrafish exposed to 125 μM neomycin and 1,000 μM TMZ.
Conclusion. TMZ attenuated neomycin-induced hair cell loss in zebrafish. The results of this study suggest that neomycin induces apoptosis, and that apoptotic cell death can be prevented by treatment with tremetazidine.
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참고문헌 (25)
참고문헌 신청
Choi J / 2013 / Protective effects of apocynin on cisplatin-induced ototoxicity in an auditory cell line and in zebrafish / J Appl Toxicol 33 (2) : 125 ~ 133
De la Cruz Rodriguez LC / 2010 / Attenuation of gentamicin-induced nephrotoxicity: trimetazidine versus N-acetyl cysteine / J Appl Toxicol 30 (4) : 343 ~ 353
El Banani H / 2000 / Changes in intracellular sodium and pH during ischaemia-reperfusion are attenuated by trimetazidine: comparison between low- and zero-flow ischaemia / C
Forge A / 2000 / Aminoglycoside antibiotics / Audiol Neurootol 5 (1) : 3 ~ 22
Guarnieri C / 1990 / Beneficial effects of trimetazidine on mitochondrial function and superoxide production in the cardiac muscle / Cardiovasc Drugs Ther 4 (Suppl 4) : 814
De la Cruz Rodriguez LC / 2010 / Attenuation of gentamicin-induced nephrotoxicity: trimetazidine versus N-acetyl cysteine / J Appl Toxicol 30 (4) : 343 ~ 353
El Banani H / 2000 / Changes in intracellular sodium and pH during ischaemia-reperfusion are attenuated by trimetazidine: comparison between low- and zero-flow ischaemia / C
Forge A / 2000 / Aminoglycoside antibiotics / Audiol Neurootol 5 (1) : 3 ~ 22
Guarnieri C / 1990 / Beneficial effects of trimetazidine on mitochondrial function and superoxide production in the cardiac muscle / Cardiovasc Drugs Ther 4 (Suppl 4) : 814
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