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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.1
- 수록면
- 355 - 362 (8page)
이용수
초록· 키워드
오류제보하기
Carcinogens result in the impairment of intercellular communication as well as intracellular
communication of normal cells. Connexin (Cx) is a main constituent protein of gap junctions that let messengers such as ions
communicate between cells. We evaluated the effect of carcinogen H₂O₂ on the expression of Cxs and gap junction intercellular
communication (GJIC) in the human keratinocyte cell line (HaCaT) and analyzed the prevention effect of green tea extracts
against H₂O₂. Materials and Method：We performed neutral red dye uptake tests to determine the optimal concentrations of
H₂O₂, green tea extracts-epicatechin (EC) and epigallocatechin-3-gallate (EGCG) in this study. To analyze the expression
change of Cxs, we performed RT-PCR, Western blot, and immunocytochemistry after 24-hour culture of HaCaT cells treated
with agents. We also evaluated GJIC quantitatively using the ‘scrape loading dye transfer (SLDT)’ technique. Results：Cx26,
Cx30, Cx31, Cx43, but not Cx29 were expressed in the HaCaT cells. H2O2 (250 uM) down-regulated Cx26 and Cx43 proteins.
In HaCaT cells treated with H₂O₂, EC (175 uM) up-regulated Cx26 and Cx43 proteins, but EGCG (50 uM) up-regulated only
Cx43 protein. Immunocytochemistry showed the decreased expression and abnormal location of Cx26 and Cx43 under H₂O₂,
and EC and EGCG (5 uM) inhibited the effect of H₂O₂, showing similar staining in the control. In SLDT, H₂O₂ down-regulated
GJIC, while EC and EGCG significantly prevented HaCaT cells from the H₂O₂-induced, down-regulation of GJIC. Conclusion：
The carcinogen, H2O2, inhibits GJIC in the keratinocyte cell line. Green tea extracts, such as EC and EGCG, prevent GJIC
inhibition in the keratinocyte cell line treated with H₂O₂, suggesting they have a potential anti-cancer properties. (Korean J
Otorhinolaryngol-Head Neck Surg 2008;51:355-62)
목차
등록된 정보가 없습니다.
참고문헌 (20)
참고문헌 신청
Bruzzone R / 1996 / The cellular internet: Online with connexins / Bioessays 18 (9) : 709 ~ 718
Cerutti PA / 1985 / Prooxidant states and tumor promotion / Science 227 (4685) : 375 ~ 381
Choung YH / 2002 / Functional study of GJB2 in hereditary hearing loss / Laryngoscope 112 (9) : 1667 ~ 1671
Choung YH / 2006 / Expression of the gap junction proteins connexin 26 and connexin 43 in human middle ear cholesteatoma / Acta Otolaryngol 126 (2) : 138 ~ 143
El-Fouly MH / 1987 / Scrape-loading and dye transfer: A rapid and simple technique to study gap junctional intercellular communication / Exp Cell Res 168 (2) : 422 ~ 430
Cerutti PA / 1985 / Prooxidant states and tumor promotion / Science 227 (4685) : 375 ~ 381
Choung YH / 2002 / Functional study of GJB2 in hereditary hearing loss / Laryngoscope 112 (9) : 1667 ~ 1671
Choung YH / 2006 / Expression of the gap junction proteins connexin 26 and connexin 43 in human middle ear cholesteatoma / Acta Otolaryngol 126 (2) : 138 ~ 143
El-Fouly MH / 1987 / Scrape-loading and dye transfer: A rapid and simple technique to study gap junctional intercellular communication / Exp Cell Res 168 (2) : 422 ~ 430
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