인간 비점막에서 Collectin의 발현

이흥만; 최진호; 이상학; 황유진; 황순재; 이진영

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자료유형: 학술저널

저자정보: 이흥만 최진호 이상학 황유진 황순재 이진영

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제45권 제10호

발행연도: 2002.1

수록면: 963 - 968 (6page)

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Background and Objectives：Collectins (surfactant protein A and D) are proteins with collagen tails and globular lectin domains that appear to play an important role in the first line of host defense in mammalians. However, it is not known if collectins are also present in human nasal mucosa. The purpose of this study was to investigate the expression of collectin proteins in human nasal mucosa and to compare the expressions of SP-A and D mRNA in the normal nasal mucosa and in chronic inflammatory nasal diseases. Materials and Method：Ten chronic rhinosinusitis patients were recruited and ten normal nasal mucosae were used as normal controls. Reverse transcriptase-polymerase chain reaction was performed to detect SP-A and SP-D mRNA. The level of collectin and GAPDH (glyceraldehyde-3-phosphate dehydrogenase) transcripts were semi-quantified with the desitometry. We have characterized the cellular localization of SP-A and SP-D protein using im-munohistochemistry. Results：SPA2/ GAPDH mRNA ratio in chronic rhinitis nasal mucosa is greater compared with that in normal nasal mucosa (p<0.05). SPA protein was expressed in the nasal epithelium and in the epithelial cells of the submucosal glands. SP-D mRNA and protein were not expressed in the nasal mucosa. Conclusion：These data provide the first evidence of the presence of collectins in the human nasal mucosa. These results suggested that up-regulation of collectin in chronic rhinosinusitis may be a protective response for the nasal mucosa. (Korean J Otolaryngol 2002;45:963-8)

#부비동염·폐계면활성제·역전사-중합효소반응·면역조직화학적 염색. #Sinusitis· Pulmonary surfactant· Reverse transcriptase-polymerase chain reaction #RT-PCR)· Imunohisto-chemistry.

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