인체비점막에서 Midkine mRNA의 발현

김은중; 오병훈; 이상학; 이창행; 최성배; 임기정; 이흥만; 김정준

추천

검색

자료유형: 학술저널

저자정보: 김은중 오병훈 이상학 이창행 최성배 임기정 이흥만 김정준

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제45권 제4호

발행연도: 2002.1

수록면: 335 - 340 (6page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Background and Objectives：A heparin-binding polypeptide called midkine is a family of secreted growth/differentiation cytokines and has a role in tumor growth by enhancing endothelial proliferation, vascular density and angiogenesis. In this respect, midkine may be involved in the pathogenesis of nasal polyposis. The aim of the present study is to evaluate the Materials and Method：The total RNA was isolated from freshly disected inferior turbinate of patients who underwent rhinoplasty and from nasal polyps of chronic rhinosinusitis patients. The expresion and distribution of midkine mRNA was investigated by reverse transcriptse- polymerase chain reaction (RT-PCR) and in situ hybridization. The midkine mRNA expression in nasal mucosa and polyps were semi-quantitatively evaluated by Southern blot hybridization. Results：The expression of midkine mRNA was identified in both normal inferior turbinate and nasal polyp. Histochemistry of In situ hybridization revealed that midkine mRNA in normal inferior turbinate was intensely expressed in the surface epithelium, submucosal glands, vascular endothelium, and inflammatory cells scatered in submucosal tissues. Midkine mRNA was expressed in the nasal polyps, many inflammatory cells and newly formed vascular endothelium, but not in the newly formed glandular epithelium. In semi-quantitative southern blot hybridization, midkine mRNAs did not have diferent expresion levels betwen inferior turbinate and nasal polyps. Conclusion：These results indicate that midkine mRNA is innately expressed in human nasal mucosa, playing a role in nasal physiology. Also, the results show that midkine may be involved in the pathogenesis of nasal polyps via angiogenesis, tissue growth, and inflamatory process. (Korean J Otolaryngol 2002;45:335-40)

#Midkine·역전사중합효소연쇄반응·In situ hybridization·하비갑개·비용. #Midkine· RT-PCR· In situ hybridization· Inferior turbinate· Nasal polyp.

참고문헌 (0)

참고문헌 신청

참고문헌이 DBpia에서 서비스 중이라면, [참고문헌 신청]을 통해 등록해보세요

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!