사람 중이점막상피세포에서 Uridine-5' Triphosphate에 의한칼슘 이동과 점액분비에 미치는 카페인의 효과

신중욱; 손은진; 박헌이; 정상호; 윤주헌; 최재영; 김성헌

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자료유형: 학술저널

저자정보: 신중욱 손은진 박헌이 정상호 윤주헌 최재영 김성헌

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제49권 제3호

발행연도: 2006.1

수록면: 263 - 265 (3page)

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Background and Objectives：Puringeric receptors and their agonists like uridine-5-triphosphate (UTP) and adenosine triphosphate (ATP), regulate mucin secretion in middle ear epithelial cells. In the present study, we examined the effects of purinergic agonists on Ca2+ influx ([Ca2+]i) in normal human middle ear epithelial (NHMEE) cells. We also examined the effect of caffeine, an inositol 1, 4, 5-triphosphate (IP3) inhibitor, on UTP induced [Ca2+]i and mucin secretion in NHMEE cells. Materials and Method：NHMEE cells were stimulated with various purinergic agonists, such as UTP, and [Ca2+]i was measured using a miniature double perfusion chamber. UTP-induced mucin secretion was quantitated by immunoblotting assay.Results：The determined order of purinergic agonist potency with respect to [Ca2+]i was ATP=UTP>2-MeSATP>ADP>> adenosine. UTP-induced mucin secretion was inhibited when the intracellular Ca2+ was removed with 2-bis (2-aminophenoxy) ethane-N, N, N, N-tetraacetic acid-acetoxymethyl ester. Caffeine suppressed UTP-induced [Ca2+]i, and but inhibited UTPinduced and constitutional mucin secretion. Conclusion：Our results suggest that caffeine may have a therapeutic effect in mucoid otitis media by suppressing mucin secretion. (Korean J Otolaryngol 2006;49:263-8)

#점액·카페인·칼슘. #Mucin·Caffeine·Calcium.

참고문헌 (18)

참고문헌 신청

/ / 5-trisphosphateelevations but are generated by a caffeine- and ryanodine-sensitiveintracellular Ca2+ store : 3005 ~ 8

/ 1996 / Nucleotide-inducedmucin release from primary hamster tracheal surface epithelial cellsinvolves the P2u purinoreceptor 9 : 542 ~ 548

/ 1997 / ATP-induced mucin releasefrom cultured airway goblet cells involves, in part, activation ofprotein kinase C 16 : 194 ~ 198

/ 1997 / Effect of caffeine on mucus secretion and agonist-dependentCa2+ mobilization in human gastric mucus secreting cells 24 (1356) : 198 ~ 206

/ 1997 / Extracellular ATP modulates ion transport viaP2Y purinoceptors in a middle-ear epithelial cell line 59 : 170 ~ 175

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