정상 사람 코점막 상피세포에서 막특이성 퓨린성 수용체의 발현

이정권; 윤주헌; 송미현; 유종범; 이세영; 김성식; 김창훈

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자료유형: 학술저널

저자정보: 이정권 윤주헌 송미현 유종범 이세영 김성식 김창훈

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제48권 제5호

발행연도: 2005.1

수록면: 606 - 614 (9page)

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Background and Objectives：Extracellular purines and pyrimidines regulate various physiological responses via cell surface receptors known as purinoreceptors, and may exert autocrine or paracrine effects on ion transport, fluid transport, ciliary beat frequency and mucin secretion. This study aims to investigate the expression patterns of such purinoreceptors found in normal human nasal epithelial (NHNE) cells. Materials and Method：In RT-PCR, the mRNAs for several P2X (P2X3, P2X4, P2X7) and P2Y (P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12) receptors were identified in NHNE cells. Functional localizations of P2 receptors were investigated by measuring [Ca2+]i increases in a membrane-specific manner using a double-perfusion chamber. Absence of the responses of -Me ATP and 2MeS-ATP excluded functionally active P2X3, P2X4, and P2Y1 receptors as far as [Ca2+]i increase was concerned. Results：Applications with ATP and UTP revealed that luminal membranes of NHNE cells express P2Y2 and P2Y6 receptors and basolateral membranes P2Y2 receptors. Expressions of P2Y2 and P2Y6 receptors in NHNE cells were further verified by the immunoblotting using specific antibodies. In addition, the results with BzATP indicated that the P2Y11 receptor may be present on the luminal side. Conclusion：The NHNE cells express functionally active P2Y2, P2Y6 and P2Y11 receptors in a membrane-specific pattern, which may play an important role in the control of mucin and fluid secretion in NHNE cells.

#퓨린성 수용체·칼슘·사람·코점막·분비물. #Purinoreceptor·Calcium·Human·Nasal mucosa·Secretions.

참고문헌 (27)

참고문헌 신청

/ 1990 / Intracellular calcium concentration of acinar cells in feline tracheal submucosal glands 259

/ 1995 / P2 purinergic receptors:Subtypeassociated signaling responses and structure 35 : 541 ~ 79

/ 1996 / Uridine nucleotide selectivity of three phospholipase C-activating P2 receptors Identification of a UDP-selective and an ATP- and UTP-specific receptor : 224 ~ 9

/ 1996 / and echanosensitivity of sinusal mucosa An in vitro study

/ 1997 / Characterization and localization of P2 receptors in rat submandibular gland acinar and duct cells 272 : 32951 ~ 5

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