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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.12
- 수록면
- 319 - 326 (8page)
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초록· 키워드
오류제보하기
Extracellular ATP elicits diverse physiological effects by binding to the G-protein-coupled P2Y receptors on the plasma membrane. In addition to the short-term effects of extracellular nucleotides on cell functions, there is evidence that such purinergic signalling can have long-term effects on cell proliferation, differentiation and death. The 3T3-L1 cell line derived from mouse embryo is a well-established and commonly utilized in vitro model for adipocytes differentiation and function. However, the distributions and roles of P2Y subtypes are still unknown in the preadipocyte. In this study, we identified the distributions and roles of P2Y subtypes in preadipocyte using Ca²? imaging and realtime PCR. ATP increased the [Ca²?]<SUB>i</SUB> in a concentration-dependent manner. ATP increased Ca²? in absence and/or presence of extracellular Ca²?. Suramin, non-selective P2Y blocker, largely blocked the ATP-induced Ca²? response. U73122, a PLC inhibitor, completely inhibited Ca²? mobilization in 3T3-L1 cells. The mRNA expression by realtime PCR of P2Y subtypes was P2Y₂:P2Y?:P2Y?=1.0:12.5:0.3. In conclusion, we showed that P2Y? receptor is a dominant purinergic receptor in preadipocytes, and multiple P2Y receptors could involve in differentiation and migration via regulating of intracellular calcium concentration.
목차
서론
재료 및 방법
결과
고찰
REFERENCES
참고문헌 (35)
참고문헌 신청
1. [학술지(정기간행물)] - Abbracchio MP / 1998 / Purinergic signaling: Pathophysiological roles / Jpn J Pharmacol 78 : 113 ~ 145
2. [학술지(정기간행물)] - Abbracchio MP / 2003 / Characterization of the UDPglucose receptor(re-named here the P2Y14 receptor) adds diversity to the P2Y receptor family / Trends Pharmacol Sci 24 : 52 ~ 55
3. [학술지(정기간행물)] - Berchtold S / 1999 / Human monocyte derived dendritic cells express functional P2X and P2Y receptors as well as ecto-nucleotidases / FEBS Lett 458 : 424 ~ 428
4. [학술지(정기간행물)] - Burnstock G / 1997 / The past, present, and future of purine nucleotides as signaling molecules / Neuropharmacology 36 : 1127 ~ 1139
5. [학술지(정기간행물)] - Burnstock G / 2007 / Purine and Pyrimidine receptors / Cell Mol Life Sci 64 : 1471 ~ 1483
2. [학술지(정기간행물)] - Abbracchio MP / 2003 / Characterization of the UDPglucose receptor(re-named here the P2Y14 receptor) adds diversity to the P2Y receptor family / Trends Pharmacol Sci 24 : 52 ~ 55
3. [학술지(정기간행물)] - Berchtold S / 1999 / Human monocyte derived dendritic cells express functional P2X and P2Y receptors as well as ecto-nucleotidases / FEBS Lett 458 : 424 ~ 428
4. [학술지(정기간행물)] - Burnstock G / 1997 / The past, present, and future of purine nucleotides as signaling molecules / Neuropharmacology 36 : 1127 ~ 1139
5. [학술지(정기간행물)] - Burnstock G / 2007 / Purine and Pyrimidine receptors / Cell Mol Life Sci 64 : 1471 ~ 1483
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UCI(KEPA) : I410-ECN-0101-2010-510-001690000