사람 정상 코점막 상피세포에서 섬모세포의 표식자로서MUC8에 관한 연구

이정권; 김경수; 윤주헌; 이욱진; 김창훈; 유종범

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자료유형: 학술저널

저자정보: 이정권 김경수 윤주헌 이욱진 김창훈 유종범

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제48권 제4호

발행연도: 2005.1

수록면: 455 - 459 (5page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

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Background and Objectives：To examine the MUC8 mRNA expression patterns according to the mucociliary differentiation ofthe normal human nasal epithelial (NHNE) cells, and to investigate the localization of the MUC8 proteins in the nasal polyps.Materials and Methods：The passage-2 NHNE cells were cultured using an air-liquid interface technique and nasal polyp specimens.On the 2, 7, 14, and 28 days after confluence, the ciliated cells were counted using cytospin slide immunostaining usingH6C5 and β-tubulin, and the MUC8 mRNA levels were determined using real-time quantitative PCR. After synthesizing the polyclonalanti-MUC8 peptide antibodies, MUC8 immunostaining was preformed using the nasal polyps. The MUC8 mRNA andprotein levels were determined with the NHNE cells treated with IL-1β (10 ng/ml for 24 hours) using RT-PCR and Western blotanalysis. Results：The increasing pattern of the number of ciliated cells as well as the MUC8 gene expression level with increasingculture time in the NHNE cells was quite similar. MUC8 was expressed in the ciliated cells of the human nasal polyps. TheMUC8 protein level as well as the mRNA level was up-regulated as a result of the IL-1β treatment. Conclusions：This study indicatesthat the MUC8 protein is expressed in ciliated cells from the human nasal epithelial cells and is up-regulated by the IL-1βtreatment. These results suggest that the MUC8 gene and protein expression levels might be used as a ciliated cell marker in thehuman nasal epithelium.

#점액유전자 8·섬모세포·코 상피세포. #MUC8Ciliated cellNasal epithelial cell.

참고문헌 (16)

참고문헌 신청

/ 1996 / Different mucins are producedby the surface epithelium and the submucosa in human trachea Identification of MUC5AC as a major mucin from the goblet cells : 319 ~ 24

/ 1997 / Allelic polymorphism and chromosomallocalization of the human oviductin gene : 702 ~ 8

/ 1997 / Chromosomal localization of a human mucin gene and cloningof the cDNA corresponding to the carboxy terminus : 232 ~ 41

/ 1997 / Identification of twoglycoforms of the MUC5B mucin in human respiratory mucus : 9561 ~ 6

/ 1999 / Control of mucin transcription by diverse injury-induced signalingpathways

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