지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한이비인후과학회 Clinical and Experimental Otorhinolaryngology Clinical and Experimental Otorhinolaryngology 제5권 제3호
- 발행연도
- 2012.1
- 수록면
- 139 - 144 (6page)
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초록· 키워드
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Objectives. Streptococcus pneumoniae is one of the most common pathogens of otitis media (OM) that exists in biofilm,which enhances the resistance of bacteria against antibiotic killing and diagnosis, compared to the free-floating (planktonic)form. This study evaluated biofilm formation by S. pneumoniae on an abiotic surface and in the middle ear cavity in a rat model of OM.
Methods. In vitro biofilm formation was evaluated by inoculation of a 1:100 diluted S. pneumoniae cell suspension in a 96-well microplate. Adherent cells were quantified spectrophotometrically following staining with crystal violet by measurement of optical density at 570 nm. The ultrastructure of pneumococcal biofilm was assessed by scanning electron microscopy (SEM). For in vitro biofilm study, S. pneumoniae cell suspensions containing 1×107 colony forming units were injected through transtympanic membrane into the middle ear cavity of Sprague Dawley rats. The ultrastructure of middle ear mucus was observed by SEM 1 and 2 weeks post-inoculation.
Results. The in vitro study revealed robust biofilm formation by S. pneumoniae after 12-18 hours of incubation in high glucose medium, independent of exogenously supplied competence stimulating peptide and medium replacement. Adherent cells formed three-dimensional structures approximately 20-30 μm thick. The in vivo study revealed that ciliated epithelium was relatively resistant to biofilm formation and that biofilm formation occurred mainly on non-ciliated epithelium of the middle ear cavity. One week after inoculation, biofilm formation was high in 50% of the treated rats and low in 25% of the rats. After 2 weeks, biofilm formation was high and low in 25% and 37.5% of rats, respectively.
Conclusion. The results imply that glucose level is important for the S. pneumoniae biofilm formation and S. pneumoniae biofilm formation may play important role in the pathophysiology of OM
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참고문헌 (25)
참고문헌 신청
[학술지(정기간
Allegrucci M / 2006 / Phenotypic characterization of Streptococcus pneumoniae biofilm development / J Bacteriol 188 (7) : 2325 ~ 2335
Baldassarri L / 2006 / Therapeutic failures of antibiotics used to treat macrolide-susceptible Streptococcus pyogenes infections may be due to biofilm formation / J Clin Mic
Block SL / 2004 / Community-wide vaccination with the heptavalent pneumococcal conjugate significantly alters the microbiology of acute otitis media / Pediatr Infect Dis J 2
Bogaert D / 2004 / Streptococcus pneumoniae colonisation: the key to pneumococcal disease / Lancet Infect Dis 4 (3) : 144 ~ 154
Allegrucci M / 2006 / Phenotypic characterization of Streptococcus pneumoniae biofilm development / J Bacteriol 188 (7) : 2325 ~ 2335
Baldassarri L / 2006 / Therapeutic failures of antibiotics used to treat macrolide-susceptible Streptococcus pyogenes infections may be due to biofilm formation / J Clin Mic
Block SL / 2004 / Community-wide vaccination with the heptavalent pneumococcal conjugate significantly alters the microbiology of acute otitis media / Pediatr Infect Dis J 2
Bogaert D / 2004 / Streptococcus pneumoniae colonisation: the key to pneumococcal disease / Lancet Infect Dis 4 (3) : 144 ~ 154
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