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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.1
- 수록면
- 24 - 28 (5page)
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초록· 키워드
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A tetracycline resistant Vibrio parahaemolyticus, capable of growing on TCBS medium containing tetracycline, was isolated from cultivated fishes. A gene responsible for the tetracycline resistance was cloned from chromosomal DNA of the V. parahaemolyticus strain using Escherichia coli KAM3, which lacks major multi-drug efflux pumps (△acrB) as host cells. The nucleotide sequence and homology analysis revealed an open reading frame (ORF) for tetracycline resistance protein (TetB). In order to characterize the antibiotic resistance of TetB originated from the V. parahaemolyticus strain, the gene was subcloned into plasmid pSTV28. The resulting plasmid was designated as pSTVTetB and transformated into E. coli KAM3. E. coli KAM3 cells harboring the recombinant plasmid pSTVTetB are able to grow on plates containing tetracycline and oxytetracycline but not doxycycline, indicating that the tetB gene confers the tetracycline- and oxytetracycline-resistance to the host cell.
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등록된 정보가 없습니다.
참고문헌 (15)
참고문헌 신청
Andersen, S.R / 1994 / Distribution of tetracycline resistance determinants among gram-negative bacteria isolated from polluted and un-polluted marine sediments / Appl. Envir
Depaola, A / 1995 / Class D and E tetracycline resistance determinants in gram-negative bacteria from catfish ponds / Mol. Cell Probes. 9 : 311 ~ 313
Depaola, A. / 1993 / Oligonu-cleotide probe determination of tetracycline resistant bacteria isolated from catfish ponds / Mol. Cell Probes. 7 : 345 ~ 348
Furushita, M. / 2003 / Similarity of tetracycline resistance genes isolated from fish farm bacteria to those from clinical isolates / Appl. Environ. Microbiol. (69) : 5336 ~
Jeanette, W. / 2002 / Genetic determinants of tetracycline resistance in Vibrio harveyi / Antimicro. Agents Chemother. 46 : 1038 ~ 1045
Depaola, A / 1995 / Class D and E tetracycline resistance determinants in gram-negative bacteria from catfish ponds / Mol. Cell Probes. 9 : 311 ~ 313
Depaola, A. / 1993 / Oligonu-cleotide probe determination of tetracycline resistant bacteria isolated from catfish ponds / Mol. Cell Probes. 7 : 345 ~ 348
Furushita, M. / 2003 / Similarity of tetracycline resistance genes isolated from fish farm bacteria to those from clinical isolates / Appl. Environ. Microbiol. (69) : 5336 ~
Jeanette, W. / 2002 / Genetic determinants of tetracycline resistance in Vibrio harveyi / Antimicro. Agents Chemother. 46 : 1038 ~ 1045
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