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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2004.1
- 수록면
- 122 - 129 (8page)
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Harmful Cochlodinium polykrikoides is a notorious harmful algal bloom (HAB) species that is causing mass mortality of farmed fish along the Korean coast with increasing frequency. We analyzed the sequence of the large subunit (LSU) rDNA D1-D3 region of C. polykrikoides and conducted phylogenetic analyses using Bayesian inference of phylogeny and the maximum likelihood method. The molecular phylogeny showed that C. polykrikoides had the genetic relationship to Amphidinium and Gymnodinium species supported only by the relatively high posterior probabilities of Bayesian inference. Based on the LSU rDNA sequence data of diverse dinoflagellate taxa, we designed the C. polykrikoides-specific PCR primer set, CPOLY01 and CPOLY02 and developed PCR detection assays for its sensitive, accurate HAB monitoring. CPOLY01 and CPOLY02 specifically amplified C. polykrikoides and did not cross-react with any dinoflagellates tested in this study or environmental water samples. The effective annealing temperature (Tp) of CPOLY01 and CPOLY02 was 67℃. At this temperature, the conventional and nested PCR assays were sensitive over a wide range of C. polykrikoides cell numbers with detection limits of 0.05 and 0.0001cells/reaction, respectively.
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참고문헌 (25)
참고문헌 신청
/ 1985 / Confidence limits on phylogenies tion : 16 ~ 24
/ 1995 / DNA extraction conditions from Porphyra perforata using LiCl. 7 : 101 ~ 107
/ 1996 / The oligonucleotide The oligonucleotide database project. database project. 62, : 3557 ~ 3559
/ 1997 / Recent harmful algal blooms and mi- tigation strategies in Korea 19, : 185 ~ 192
/ 1998 / Physiology and bloom dynamics of toxic Alexandrium species, with emphasis on life cycle transitions. In: Physiological Ecology of Harm- ful Algal Blooms, Anderson, D
/ 1995 / DNA extraction conditions from Porphyra perforata using LiCl. 7 : 101 ~ 107
/ 1996 / The oligonucleotide The oligonucleotide database project. database project. 62, : 3557 ~ 3559
/ 1997 / Recent harmful algal blooms and mi- tigation strategies in Korea 19, : 185 ~ 192
/ 1998 / Physiology and bloom dynamics of toxic Alexandrium species, with emphasis on life cycle transitions. In: Physiological Ecology of Harm- ful Algal Blooms, Anderson, D
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