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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 65 - 71 (7page)
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The functional utility of a transgenic marine medaka Oryzias dancena strain carrying the red fluorescent protein (RFP) gene driven by an endogenous choriogenin H (chgH) promoter was evaluated for its ability to detect waterborne 17α-ethinylestradiol (EE2), a synthetic estrogen derivative. The chgH:rfp transgenic marine medaka larvae showed an age-dependent tendency in the efficiency of EE2-mediated transgene expression, in which transgenic larvae older than 6 days post-hatching displayed a more effective response in their transgene expression to EE2 than did younger hatchlings. During experimental exposures to high concentrations of EE2 (200 to 1,000 ng/L), the transgenic responses in the hatchlings were broadly dose- and duration-dependent. With exposures using lower doses of EE2 (25, 50 and 100 ng/L), EE2-induced transgenic RFP was also observed in the transgenic larvae, although the lower doses required exposure of longer duration. Under the EE2 exposure and microscope assay conditions used in our study, transgenic marine medaka larvae exhibited a similar degree of EE2-mediated RFP phenotype expression at various salinity levels (0, 15 and 30 ppt).
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참고문헌 (20)
참고문헌 신청
Aris AZ / 2014 / Occurrence of 17α-ethynylestradiol(EE2)in the environment and effect on exposed biota : a review / Environ Intl 69 : 104 ~ 119
Blewett T / 2013 / The effects of temperature and salinity on 17α-ethinylestradiol uptake and its relationship to oxygen consumption in the model euryhaline teleost(Fundulus
Chen H / 2010 / Generation of a fluorescent transgenic zebrafish for detection of environmental estrogens / Aquat Toxicol 96 : 53 ~ 61
Cho YS / 2013 / Characterization of estrogen-responsive transgenic marine medaka Oryzias dancena germlines harboring red fluorescent protein gene under the control by endogen
Filby AL / 2007 / Gene expression profiles revealing the mechanisms of anti-androgen-and estrogeninduced feminization in fish / Aquat Toxicol 81 : 219 ~ 231
Blewett T / 2013 / The effects of temperature and salinity on 17α-ethinylestradiol uptake and its relationship to oxygen consumption in the model euryhaline teleost(Fundulus
Chen H / 2010 / Generation of a fluorescent transgenic zebrafish for detection of environmental estrogens / Aquat Toxicol 96 : 53 ~ 61
Cho YS / 2013 / Characterization of estrogen-responsive transgenic marine medaka Oryzias dancena germlines harboring red fluorescent protein gene under the control by endogen
Filby AL / 2007 / Gene expression profiles revealing the mechanisms of anti-androgen-and estrogeninduced feminization in fish / Aquat Toxicol 81 : 219 ~ 231
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