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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
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- 학술저널
- 저자정보
- 발행연도
- 2016.1
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- 1 - 7 (7page)
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Background: The transgenic approach using estrogen-responsive regulator in fish has been given much attention as a potential means to detect and/or address estrogen-related aquatic pollutions. In order to address the development stage- and reproduction status-dependent expression patterns of the chgH-rfp transgene (red fluorescent protein transgene driven by choriogenin H promoter) in marine medaka Oryzias dancena, naturally occurring red fluorescent protein (RFP) signals under non-exposed conditions as well as the transgenically induced RFP signals under estrogen-exposed conditions were assayed.
Results: Female transgenics begun to show naturally occurring RFP signals from the age of 7 weeks post hatching (WPH) without experimental estrogen exposure. Afterward, these RFP signals in female transgenics became robust with the progress of ovarian maturation. On the other hand, male transgenics did not show any naturally occurring RFP signal under non-exposed conditions irrespective of developmental stages and maturation statue. Upon exposures using estradiol-17β (E2) and 17α-ethinylestradiol (EE2), RFP signals were significantly induced specifically in the livers of transgenic males.
Conclusions: Male chgH-rfp transgenics were able to keep the “off” state of RFP expression during their entire life cycle unless exposed to exogenous estrogens. Owing to their tight regulation capability of estrogen-responsive transgene, transgenesis of chgH-rfp in male marine medaka could offer a useful model system for future ecotoxicogenomic studies regarding estrogenicity-related issues in aquatic and marine environments.
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참고문헌 (22)
참고문헌 신청
Bogers R / 2006 / Estrogenic endpoints in fish early life-stage tests : luciferase and vitellogenin induction in estrogen-responsive transgenic zebrafish / Environ Toxicol Chem 25:241~247
Chen X / 2008 / Choriogenin mRNA as a sensitive molecular biomarker for estrogenic chemicals in developing brackish medaka(Oryzias melastigma) / Ecotoxicol Environ Saf 71:200~208
Chen H / 2010 / Generation of a fluorescent transgenic zebrafish for detection of environmental estrogens / Aquat Toxicol 96:53~61
조영선 / 2010 / Spawning Performance, Embryonic Development and Early Viability under Different Salinity Conditions in a Euryhaline Medaka Species, Oryzias dancena / 한국어류학회지 22(1):25~33
Cho YS / 2011 / Functional ability of cytoskeletal β-actin regulator to drive constitutive and ubiquitous expression of a fluorescent reporter throughout the life cycle of transgenic marine medaka Oryzias dancena / Transgenic Res 20:1333~1355
Chen X / 2008 / Choriogenin mRNA as a sensitive molecular biomarker for estrogenic chemicals in developing brackish medaka(Oryzias melastigma) / Ecotoxicol Environ Saf 71:200~208
Chen H / 2010 / Generation of a fluorescent transgenic zebrafish for detection of environmental estrogens / Aquat Toxicol 96:53~61
조영선 / 2010 / Spawning Performance, Embryonic Development and Early Viability under Different Salinity Conditions in a Euryhaline Medaka Species, Oryzias dancena / 한국어류학회지 22(1):25~33
Cho YS / 2011 / Functional ability of cytoskeletal β-actin regulator to drive constitutive and ubiquitous expression of a fluorescent reporter throughout the life cycle of transgenic marine medaka Oryzias dancena / Transgenic Res 20:1333~1355
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